23 what is the expected sizes of the restriction

This preview shows page 2 - 4 out of 4 pages.

Which restriction enzyme has a unique recognition site within the Mag1 coding region? 2.3) What is the expected sizes of the restriction fragment/s generated from a digestion of this recombinant plasmid with SalI? 2.4) What is the expected sizes of the restriction fragment/s generated from a double digestion of this plasmid with both KpnI and BglII?
Image of page 2

Subscribe to view the full document.

BIOL2262 2019 Assignment 1: Recombinant DNA Technology 3 3. Sub-cloning strategy: Describe how you would sub-clone the KpnI/SpeI fragment from the recombinant clone in part 2 into the vector pBluescript SK + (map of the vector below). * Include how you would form the recombinant molecule, amplify recombinant plasmids in bacteria and how you would select for the recombinant clone in E. coli . Note: You do not have the sequence or any primers so you cannot use PCR. f1 (+) ori MCS lacZ' P lac ampicillin pUC ori Kpn I Sac I pBluescript II SK (+) 3.0 kb pBluescript II SK (+/–) Multiple Cloning Site Region (sequence shown 598–826) Sac I BstX I ...GGTATCGATAAGCTTGATATCGAATTCCTGCAGCCCGGGGGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTC... EcoR I EcoR V Sac II Spe I Sma I Xba I Pst I Hind III Bsp106 I Cla I BamH I Not I Eag I TTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGCGAATTGGGTACCGGGCCCCCCCTCGAGGTCGAC... Acc I Kpn I Xho I Hinc II BssH II EcoO109 I Dra II Apa I Sal I T7 Promoter T7 primer binding site ...CAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCC
Image of page 3
Image of page 4

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern