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Procedure a broth culture 1 one tube was labeled with

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Procedure:A) Broth Culture1One tube was labeled with "Control" and left uninoculated. 2The cap or plug was removed from one tube and a small amount of dirt orother foreign material was added to it. The plug was replaced.
3After flaming the wire loop and the third tube of broth, the pure culture ofEscherichia coliwas inoculated in the third tube. The procedure was repeatedby inoculate Micrococcus luteusto the fourth tube.4The four tubes were incubated at room temperature for 2 days.B) Agar Slope1Three tubes of nutrient agar were melted in boiling water. They were cooled inan inclined position.2When the medium is cold and solid, one surface was inoculated withEscherichia coliby using needle. The needle was moved gently on the agarsurface from the bottom to top. The surface of second tube was inoculatedwith Micrococcus luteus. The third tube was left uninoculated as a controltube.3Both culture tubes were incubated at room temperature for 2 days.C) Pure Culture TechniquesI) Streak Plate1Two nutrient agar plates were provided. By using inoculation loop, the mixedculture on the two plates were streaked carefully. First plate was streakedusing the first method and another plate was streaked using the secondmethod. Aseptic technique was used:a) flame loopb) remove cap, flame lipc) remove sampled) flame lip, recap tubee) lift lidf) streakg) coverh) flame loop2The Petri plates were incubated in inverted position at room temperature for 2days.II) Pour Plate1Three tubes of melted nutrient agar were prepared. This agar was cooled to 45- 47 .
2One loopful of a mixed microbial culture was transferred to the first tube ofmelted agar. The inoculum was mixed well by thumping the tube withforefinger.3Two loopfuls of the mixed inoculum and agar from the first tube weretransferred to a second tube of melted agar and mixed well.4Three loopfuls from the second tube agar was transferred to the third tube ofagar and mixed thoroughly.

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Term
Summer
Professor
N/A
Tags
Petri dish, Micrococcus luteus, M luteus, broth culture

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