Brunskill E Mafb EGFP BAC transgenic mice were utilized to isolate the podocyte

Brunskill e mafb egfp bac transgenic mice were

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Potter S. Brunskill E Mafb-EGFP BAC transgenic mice were utilized to isolate the podocyte cells from the glomerlus from adult kidneys. The podocyte cells were isolated from the kidney using a combination of Collagenase A digestion. sieving and tyrpsinization. RNA was isolated and the gene expression profiles were determined by microarrays. Series GSE33714 Prim M-Pod GSM833242 Podocyte cells RPMI Replicate 1 GSM833244 Podocyte cells RPMI Replicate 2 GPL13730 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [CDF: MoGene10stv1_Mm_ENTREZG_1 4.1.0] Primary cultures of glomerular parietal epithelial cells or podocytes with proven origin Kabgani N, Grigoleit T, Schulte K, Sechi A, Sauer- Lehnen S, Tag C, Boor P, Kuppe C, Warsow G, Schordan S, Mostertz J, Chilukoti R, Homuth G, Endlich N, Tacke F, Weiskirchen R, Fuellen G, Endlich K, Floege J, Smeets B, Moeller MJ Total RNA for genechip analysis was derived from: 1) Primary parietal cells isolated by FACS sorting of glomeruli cultivated in 1a) RPMI media 1b) EGM-MV media 2) Primary podocyte cells isolated by FACS sorting of glomeruli cultivated in 2a) RPMI media 2b) EGM-MV media. Series GSE41523 Diff E-Pod GSM1018531 Differentiated mouse podocyte Replicate 1 GSM1018532 Differentiated mouse podocyte Replicate 2 GSM1018533 Differentiated mouse podocyte Replicate 3 GPL13730 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [CDF: MoGene10stv1_Mm_ENTREZG_1 4.1.0] Differentiated mouse podocytes (SVI) Schordan E, Schordan S, Warsow G, Chilukoti RK, Homuth G, Fuellen G, Endlich N, Endlich K Transcriptomes of differentiated cells of the conditionally immortalized mouse podocyte cell line SVI.
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  • Spring '16
  • Nephron, Glomerulus, Potter S. Brunskill E

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