3.How does one improve resolution? What does a numerical aperture N.A.) indicate? What is the relationship between resolving power and numerical aperture? 4.What is a stage micrometer? How is it different from an ocular reticle? How are a stage micrometer and ocular reticle used together?
5.Report in the table below for each of the objectives (4x 10x, 40x, & 100x), the corresponding N.A, total magnification, and the calculated theoretical resolution using Abbe’s equation (show your work, indicate the wavelength of light chosen). 6. Based upon the steps you performed to determine the size of the Parameciumspecimen using the ocular reticle and the stage micrometer, list a set of brief instructions to determine the actual size (in SI units) of any object. Report the calibration of reticle units you determined for each objective in the table below as well.Start with the scanning lens looking at the stage micrometer. Use the ocular retile to calculate how many reticle units equaled 1 mm (or the most accurate frame of reference you can make).
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- Spring '08
- Biology, Bone marrow, white blood cell