Vibrio splendidus Kidney anterior liver spleen and fluid from within the

Vibrio splendidus kidney anterior liver spleen and

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Vibrio splendidus Kidney (anterior), liver, spleen and fluid from within the peritoneal cavity contained bacterial populations, which grew on TSA supplemented with 2% (w/v) sodium chloride and on TCBS with incubation at 22°C for 48 h (Lupiani et ai, 1989). Jensen et al. (2003) used nutrient agar supplemented with 5% (v/v) sheep blood and 1.5% (w/v) NaCl, which was inoculated with kidney tissue and incubated aerobically at 15°C for up to 7 days. Vibrio tapetis Jensen et al. (2003) used nutrient agar supplemented with 5% (v/v) sheep blood and 1.5% (w/v) NaCl, which was inoculated with kidney tissue and incubated aerobically at 15°C for up to 7 days. Vibrio vulnificus Use of standard bacteriological media, such as seawater agar and TSA supplemented with sodium chloride, and incubation for up to 7 days at 20-25° C is sufficient to obtain cultures of the pathogen (Muroga et ai, 1976a, b; Nishibuchi and Muroga, 1977, 1980; Nishibuchi et al, 1979, 1980). MISCELLANEOUS PATHOGENS ^^Candidatus Arthromitus' Isolation of bacteria with the morphological characteristics of "Candidatus Arthro- mitus" was not achieved (Michel et al., 2002a).
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174 Bacterial Fish Pathogens Streptobacillus Brain heart infusion supplemented with 10% (v/v) foetal calf serum and 1% (w/v) sodium chloride with incubation for 10 days at 22° C was used to recover the novel Streptobacillus-likQ organism (Maher et al, 1995). UNIDENTIFIED GRAM-NEGATIVE ROD The intracellular, Gram-negative cocco-bacilli required serum or blood for growth, which was accomplished on 7% (v/v) horse blood agar and 10% (v/v) foetal calf serum medium after 4-14 days incubation at 15 or 22°C (Palmer et ai, 1994). The unnamed organism associated with Varracalbmi grew at 22° C on 5% (v/v) citrated bovine blood agar supplemented with 2% (w/v) NaCl from inocula obtained from dermal lesions, kidney and liver (Valheim et al, 2000). APPENDIX 5.1 MEDIA USED FOR THE ISOLATION AND GROWTH OF BACTERIAL FISH PATHOGENS Anderson and Conroy's medium for Sporocytophaga-Wke organisms 5.0% (w/v) 0.1% (w/v) 0.1% (w/v) 0.9% (w/v) pH This medium is enzymic digest of fish muscle peptone yeast extract agar 7.0 prepared in seawater. Bootsma and Clerx's medium for Flavobacteviiim coliimnare 0.05% (w/v) 0.05% (w/v) 1.0% (w/v) pH casitone yeast extract agar 8.0 Brewer's thioglycollate medium 0.1% (w/v) 0.2% (w/v) 0.5% (w/v) 0.5% (w/v) 0.5% (w/v) 0.11% (w/v) 0.0002% (w/v) 0.1% (w/v) pH SteriHse at 121° Lab-lemco yeast extract peptone dextrose sodium chloride sodium thioglycollate methylene blue agar No. 1 7.2 C/15min.
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Isolation/Detection 175 Charcoal agar; for the growth of Renibactevium salmoninarum 1.0% (w/v) peptone 0.05% (w/v) yeast extract 0.1% (w/v) L-cysteine hydrochloride 0.1% (w/v) activated charcoal 1.5% (w/v) agar pH 6.8 Sterilise at 12TC for 15 min (the charcoal may be placed in dialysis tubing prior to sterilisation in order to obtain a clear broth medium). Columbia agar 2.3% (w/v) special peptone 0.1% (w/v) starch 0.5% (w/v) sodium chloride 1% (w/v) agar No. 1 pH 7.3 Sterilise at 12rc/15min.
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