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Lab__3b_Identification_of_Unknown_Bacteria_-_Part_II

Bright pink staining acid fast cells af cell are

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Endospore Stain Distinguishes between two things: - endospores, which stain green - vegetative cells, which stain pink Some bacteria produce endospores, dormant, highly-resistant structures that can survive environmental extremes (desiccation, heat, harmful chemicals). Most notable genera: Bacillus and Clostridium Endospores cannot be stained by normal staining procedures because their walls are practically impermeable. Endospore stain uses heat to drive the primary stain, (malachite green) into the endospore. After cooling, the sample is decolorized with water and counter stained with safranin. Results in green stained endospores and red-colored vegetative cells . +u - E ENDOSPORE STAINING PROCEDURE Malachite Green (5 min heat) > rinse Safrinin (1 min) > rinse & blot dry From the Virtual Microbiology Classroom on ScienceProfOnline.com Watch video of How to Do an Endospore Stain
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Dichotomous Key Simple Stain Cocci Bacilli Gram Stain Gram negative cocci Gram positive cocci Mannitol Salt yellow pink Staphylococcus aureus Staphylococcus epidermis Gram Stain Gram negative bacilli Gram positive bacilli MacConkey’s No color change Salmonella pullorum Pink colonies E. coli Enterobacter aerogenes Acid Fast stain Acid Fast Mycobacterium tuberculosis Not acid fast Endospore stain Forms endospores Bacillus subtilus
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bright pink staining Acid fast cells AF Cell are considered...

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