You will always be looking through the ocular and

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You will always be looking through the ocular and objective lens simultaneously, so multiply ocular magnification x objective power to calculate the Total Magnification (xTM). Rotary nosepiece of your microscope has four objective lenses attached. Shortest lens (red band) should have been pointing down when your scopes were last put away. Magnification & The Objective Lenses Image: Microscope objective lenses, T. Port From the Virtual Microbiology Classroom on ScienceProfOnline.com
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Scanning Power Objective Lens Image: Microscope objective lenses, T. Port Red band around it. Magnifies objects 4x. Q: What is the Total Magnification? ____ TM We will only use this lens in today’s lab. It is not useful for looking at bacteria. From the Virtual Microbiology Classroom on ScienceProfOnline.com
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Low Power Objective Lens Has yellow band around it. Magnifies objects 10x. Q: What is the Total Magnification? ____ TM Start with this lens when looking at a bacterial smear. Q: What does the term parfocal mean? Image: Microscope objective lenses, T. Port From the Virtual Microbiology Classroom on ScienceProfOnline.com
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High Dry Objective Lens Has blue band around it. Magnifies objects 40x. Q: What is the Total Magnification? ____ TM Switch to this lens after getting your specimen in focus at 100xTM. Image: Microscope objective lenses, T. Port From the Virtual Microbiology Classroom on ScienceProfOnline.com
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Many microscopic images in your textbook were obtained using electron microscopes. Electron beam wavelengths are shorter than light wavelengths, so better resolving power. Q: What is resolution , in the context of microscopy? Transmission Electron Microscope (TEM): 2-D image Scanning Electron Microscope (SEM): 3-D image Microscopy : Electron Microscopes Images: Poliovirus, taken with TEM, PHIL #1875, Blood cells , taken with SEM, National Cancer Institute; Scanning electron microscope, Geological Survey of Israel laboratory.
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