Addition of DNS at the end of the incubation period stopped the reaction by

Addition of dns at the end of the incubation period

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6. Addition of DNS at the end of the incubation period stopped the reaction by denaturing sucrase. Explain why it is important to denaturesucrase before measuring product concentration.ResultsTable 4: Effect of pH on Sucrase ActivityOptical Densityph 3ph 4ph 5ph 6ph 7ph 8ph 910.1710.3360.6440.9210.8140.4060.16420.1840.3220.6670.9210.8110.4180.19230.1700.3120.6540.9640.8230.3930.167average0.1750.3230.6550.9350.8160.4060.174Effect of pH on Sucrase Activity1. State the optimum pH for sucrase activity and describe how sucrase activity changes at more acidic and more alkaline pH values.2. Was the rate of increase of sucrase activity higher at a pH of 8.5 or a pH of 5.5?Table 5: Effect of Temperature on Sucrase ActivityOptical Density10 °C (50 °F)20 °C (68 °F)30 °C (86 °F)40 °C (104 °F)50 °C (122 °F)60 °C (140 °F)70 °C (158 °F)10.0080.2790.7950.9660.9360.8080.62720.0060.2980.7700.9570.9040.8090.60630.0080.2960.7930.9320.9430.8480.613average0.0070.2910.7860.9520.9280.8220.615Effect of Temperature on Sucrase Activity3. State the optimum temperature for sucrase activity and describe how sucrase activity changes at lower and higher temperatures.4. Was sucrase activity higher at 25 °C or 55 °C?Laboratory Report/ Casey Fetch/ Enzyme Activity/ Michael Allar/ 11.12.2018/ Page [2] of [4]
Table 6: Effect of Sucrose Concentration on Sucrase ActivityOptical Density35 g/L30 g/L25 g/L20 g/L15 g/L10 g/L5 g/L0 g/L11.0081.0120.9480.9370.8320.7720.5230.00221.0120.9970.9440.8920.8250.7770.5220.00130.9841.0010.9450.8990.8620.7350.5280.001average1.0001.0000.9460.9090.8400.7610.5240.001Effect of Sucrose Concentration on Sucrase Activity5. State how sucrase activity changes with increasing sucrose concentration.

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