About 1 drink per hour so whatever is excess to that

about 1 drink per hour so whatever is excess to that one drink stays in your blood, causing a
person to get drunk. When a person operates a motor vehicle under these conditions they are a
risk to not only themselves, but the innocent people around them.
Materials
 Ethanol
 N-propanol
 Sodium chloride
 Bovine whole blood
 Unknown sample
 10% bleach
 GC vials and caps (24)
 GC syringes (2)
 100 mL volumetric flask (15)
 50 mL volumetric pipettes
 100 uL fixed micropipette and tips
 Parafilm
 250 mL volumetric flask
 50-100 mL of DI water
 Burette
 Sharpie

 Labeling paper
 Oven
 1 mL air tight syringe
Toxicity/ Safety
Ethanol (CH
3
CH
2
OH)
MW: 46.07
g
mol
IUPAC: ethanol
Odor: pleasant
BP: 78.2°C
MP:-114.1°C
 Highly flammable liquid and vapor
 Proper PPE must be worn when handling: gloves, goggles, lab coat and close-toed shoes
N-propanol (C
3
H
8
O)
MW: 60.1
g
mol
IUPAC: propan-1-ol
Odor: alcohol-like
BP: 97.2°C
MP: -126.1°C
 Highly flammable liquid and vapor

 Causes serious eye damage
 May cause drowsiness or dizziness
 Proper PPE must be worn when handling: gloves, goggles, lab coat, and close-toed shoes
Sodium chloride (NaCl)
MW: 58.44
g
mol
IUPAC: sodium chloride
BP: 1465°C
MP: 800.7°C
 Causes serious eye irritation
 Proper PPE must be worn when handling: gloves, goggles, lab coat and close-toed shoes
Bleach
 Causes serious eye damage
 Very toxic to aquatic life with long lasting effects
 Proper PPE must be worn when handling: gloves, goggles, lab coat, and close-toed shoes
Water (H
2
O)
MW: 18.015
g
mol
IUPAC: oxidane
BP: 100°C

MP: 0°C
 Essential for life
 Proper PPE must be worn when handling: gloves, goggles, lab coat, and close-toed shoes
Procedures
1. Prepare a solution of 0.02g/dL n-Propanol in saline solution in 100mL volumetric flask
and cover with parafilm and store at 2-8°C.
2. `To create the stock solution make 0.631g/dL ethanol stock solution in 250mL volumetric