Dna methylation patterns are reversable endogenous

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DNA methylation patterns areREVERSABLEEndogenous pathways control theestablishment & removal of DNAmethylationDNA methylation patterns can beinfluenced by exogenous sourcesEnvironmental factors (diet,smoking, etc.)Medical compounds (“drugable”)Methylation ofDNAon (CpG islands) leads to gene silencingMethylation ofhistone tailslead to both activation (H3K4me3, H3K4me1+H3K27ac) andsilencing (H3K9me3)Altering the DNA methylation patterns can lead to diseased states due to deviation fromnormal gene regulation (the DNA sequence is not altered = epigenetic)
1a) Use A, B, C, etc, to fill in the boxes above the gene schematic below, to indicate the position inthe gene of each of the following important sequence elements. (Some could be present at morethan one place; not all boxes are used).This is the closest position to thetranscription start site, so it must a) bepart of the promoter and b) contain theconsensus elements necessary for thegeneral transcription factors to bind inorder for the PIC to assemble.If we are considering a TATAA-boxdependent promoter, we expect TBP(within the TFIID complex) to bind to theTATAA consensus element using its DNAbinding domain. After TFIID bindingthrough TBP and TAFs, TFIIB and TFIIHwill associate with TFIID and facilitateRNAPII recruitment and phosphorylation.G
1a) Use A, B, C, etc, to fill in the boxes above the gene schematic below, to indicate the position inthe gene of each of the following important sequence elements. (Some could be present at morethan one place; not all boxes are used).The +1 indicates the transcription startsite within the promoter (The TSS is notincluded as a choice here, so we willleave the box blank).
1a) Use A, B, C, etc, to fill in the boxes above the gene schematic below, to indicate the position inthe gene of each of the following important sequence elements. (Some could be present at morethan one place; not all boxes are used).This must be the AUG (formyl-Met/Meti)codon. If you look at “Protein X” at thebottom, the N-terminus lines up with themiddle of the first exon, which is a goodindicator that this location is wheretranslation was initiated.B
1a) Use A, B, C, etc, to fill in the boxes above the gene schematic below, to indicate the position inthe gene of each of the following important sequence elements. (Some could be present at morethan one place; not all boxes are used).This must be the 5’ splice site/donor siteof exon 1 for 2’-5’ phosphodiester bondformation via nucleophilic attack fromthe 2’OH of the branch pointA

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