BIOS242_Week3LabReport_MIKAELLA_VILLAR.docx

Of glucose broth with oil turned yellow indole

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OF glucose broth with oil + Turned yellow Indole production test + Red ring formed around media Malonate + Turned blue Bacterium identified as: Shigella dysenteriae Assigned bacterium was: Shigella dysenteriae
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2 Discussion and Conclusion Based on your results, is your bacteria sample a strict aerobe, strict anaerobe, facultative anaerobe, or microaerophile? Why? Look at your results from the OF glucose and OF glucose with oil tests. Strict anaerobe because there is no oxygen during fermentation and the media turned yellow indicating the presence of acids. The oil seals the media from oxygen and the media still turned yellow. How important is it to add the Kovac’s reagent in the Indole utilization test? What is responsible for the color change observed in a positive test? You can read more about this test in the Test Media Reference in VUMIE2012. Click the M? button. Kovac’s is added to tryptone broth. A positive test’s result shows a red ring production in the broth. This red ring indicates the production of indole. Did the bacteria grow in the tryptone or malonate media? Why or why not? Remember, you can tell whether they grew if the culture was turbid (cloudy). Bacteria grew both media. For the media used in this portion of the experiment, are the media complex or synthetic? Are the media selective, differential, both, or neither? Identify the carbon and nitrogen source for each medium.
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