replicated and proper handling and washing of cuvette and the use of

Replicated and proper handling and washing of cuvette

This preview shows page 5 - 6 out of 6 pages.

replicated and proper handling and washing of cuvette and the use of spectrophotometer was implemented.
Image of page 5

Subscribe to view the full document.

The covering of the reagents and samples should also be observed to preserve the purity of the solution. Although there are restrictions to spectrophotometry, it is still widely used and has its own applications to various fields of science and technology. Environmental applications are mostly employed on analysis of water and wastewater. It is also used to determine the organic constituents of water and significant airborne pollutants. In the clinical field, spectrophotometry is applied in the analysis of drugs and narcotics. It is also used to determine the blood alcohol using the Breathalyzer test. All in all, the application of this experiment is mainly for the analysis of industrial processed samples (Harvey, 395-398). CONCLUSION The analysis of Cu 2+ concentration using UV-Vis spectrophotometry resulted to very accurate and precise results. The analytical wavelength was found to be 596nm by scanning the most concentrated solution. The equation of the calibration curve was derived as y = 0.001x – 0.005 (7). The concentration of the sample is 2574 ppm, this gives a very small deviation of 2.94%. The relative standard deviation is zero since the absorbances are all the same. The confidence interval is just the concentration of the analyte itself, which is 2574 ppm. The experiment could have yielded better results if replication of procedures and data was followed. There could be a great improvement if the working environment was maintained at standard conditions. However, the experiment still produced very accurate and precise results, which proves the efficiency of spectrophotometry as a mode of analysis and a best example of separation science. REFERENCES Brown, T. et al. 2004. Chemistry: The Central Science. Pearson. USA Harvey, David. 2000. Modern Analytical Chemistry. Mcgraw Hill. USA Skoog D.A., West D.M., Holler F.J., 1996. Fundamentals of analytical chemistry, 7 th Ed. Saunders College Publishing, USA Jeffrey, G.H. 1989. Vogel’s Textbook Of Quantitative Chemical Analysis 5 th Ed. Longman & Scientific Technical. USA Christian, G. 2004. Analytical Chemistry. John Wiley and Sons. USA
Image of page 6

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern

Ask Expert Tutors You can ask 0 bonus questions You can ask 0 questions (0 expire soon) You can ask 0 questions (will expire )
Answers in as fast as 15 minutes