Volume of CSF minimum of 5 to 10 mL is recommended Sterile plastic screw cap

Volume of csf minimum of 5 to 10 ml is recommended

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Volume of CSF :minimum of 5 to 10 mL is recommended Sterile, plastic, screw cap tubes. 1 st : Chemistry : Glucose+ protein 2 nd : Microbiology ( Gram staining and culture) 3 rd : Hematology (WBCs and RBCs) Transport immediate processing ( STAT )
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Meningitis Causes a. Bacterial ( Purulent ): 1. Meningococci( Neisseriae meningitidis 2. Pneumococci( Streptococcus pneumoniae ) 3. Hemophilus influenzae-b is most common in child (1M-6 ys) Viruses(Aseptic) Fungi and Mycobacterium tuberculosis may cause chronic meningitis
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Gastric Lavage Nurse role : sample c ollection for determination of TB Applied for children who have swallowing sputum. The specimen is collected before breakfast Gastric juice is having harmful effect on acid-fast bacilli If delay is inevitable then neutralize it with sodium hydroxide and keep it in refrigerator till processed. Specimen is allowed to stand in a refrigerator.
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Blood Culture Sample : blood (sterile) Nurse role : sample collection (open system and close system ) Time: before administration of antibiotic s , during fever phase. Volume : 1:5-1:10(blood to media ratio) Adult:8-10 ml Pediatric: 1- 5ml Container: Two bottles aerobic and anaerobic broth Anticoagulant: Sodium polyanethol sulfonate
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SPS will deactivate phagocytes, complement system, antibodies Resin bead will deactivate antibiotics if already administrated (automated system) Media for aerobic bottle : trypticase soy broth, brain- heart infusion broth, supplemented peptone Media for anaerobic bottle : thioglycolate broth. Aerobic and anaerobic blood culture bottles
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Blood culture Site preparation: Select the vein, the skin site is swabbed with 70% isopropyl alcohol and an antiseptic is then applied to kill surface and subsurface bacteria( iodine tincture or chlorhexidine); Follow the manufacturer’s recommendation regarding exposure time. Clean the top of the bottle with 70% ethanol Inoculate into aerobic bottle first then the anaerobic bottle to remove air bubbles collected in the syringe.
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Septicemia/bacteremia Septicemia : Presence of bacteria in blood with multiplication Toxins may be produced Number of bacteria is high Appearance of signs and symptoms Bacteremia : Presence of bacteria in blood No toxins and numebr of bacteria is low No symptoms or signs) Etiology of septicemia S.aureus(acute), Neisseriae meningitdtis(chronic) Salmonella typhi
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  • Fall '19
  • Bacteria, specimen collection, Wound infection, sputum,  Express

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