Inoculum preparation nine dilutions of each drug

This preview shows page 2 - 4 out of 4 pages.

Inoculum preparation Nine dilutions of each drug (white tea extract mouthwash, chlorhexidine mouthwash, and distilled water) were done with BHI for minimal inhibitory concentration. In the first tube, 20 µ l of the drug was added into the 380 µ l of BHI broth. For dilutions, 200 µ l of BHI broth was put in the other 9 tubes separately. Then, from the first tube, 200 µ l was put into the first tube containing 200 µ l of BHI broth. This was said to be as 10:1 dilution. From 10:1 diluted tube 200 µ l was put to the second tube to make 10:2 dilution. The serial dilution was repeated up to 10:9 dilution for each drug. From the maintained stock cultures of required organisms, 5 µ l was taken and was put into 2 ml of BHI broth. In each serially diluted tube, 200 µ l of above culture suspension was added. The tubes were incubated for 24 h and observed for turbidity. Statistical analysis After the indices were calculated and the mouthwash order was decoded, further testing was done with SPSS version 17 software (SPSS Inc., Chicago, IL, USA). The mean was calculated for each mouthwash. The analysis of variance (ANOVA) was done. Differences between the mouthwash solutions and distilled water were determined via the Bonferroni multiple comparison test. RESULTS Forty-five subjects, 11 males, and 34 females, completed the study. In all groups, PI showed significant ( P <0.01) increase from the baseline to the 5 th day. The in vivo results demonstrated that the mean PI values were the highest for the distilled water mouthwash (2.55) and the least for [Downloaded free from on Friday, September 15, 2017, IP: 36.79.172.174]
Image of page 2
Mitra, et al .: White tea mouthrinse Journal of Indian Society of Periodontology 3 chlorhexidine mouthwash (1.94). The mean PI value for distilled water (2.55) was higher than the white tea group (2.21). ANOVA showed a statistically significant difference between the PI scores ( P = 0.001). Differences between the individual mouthwashes and distilled water, determined through the Bonferroni multiple comparison test, showed significantly less plaque regrowth in case of both chlorhexidine ( P =1.94) and white tea ( P = 2.21) as compared to distilled water. The results of the ANOVA test are given in Table 1 while those of the Bonferroni multiple comparison test are given in Table 2. There was no burning sensation or taste alteration reported by any of the subjects at the end of the study period. In addition, there was no evidence of gingival epithelial desquamation on intraoral examination in any of the subjects. The in vitro study showed that Pg, Pi, and Aa was inhibited at the concentration of 1%. DISCUSSION This present study evaluated the antiplaque effects of a new white tea mouthwash and also aimed to evaluate whether the mouthwash had any adverse effects. White tea mouthwash was compared with distilled water and 0.2% chlorhexidine solution. The study design (4-day plaque regrowth study) has been used in many studies and is a standard method for testing the plaque-reducing effect.
Image of page 3
Image of page 4

You've reached the end of your free preview.

Want to read all 4 pages?

  • Fall '19
  • White Teeth, Mouthwash, Green tea, Camellia sinensis, Chlorhexidine, Journal of Indian Society of Periodontology

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern

Stuck? We have tutors online 24/7 who can help you get unstuck.
A+ icon
Ask Expert Tutors You can ask You can ask You can ask (will expire )
Answers in as fast as 15 minutes
A+ icon
Ask Expert Tutors