If it ʼ s a petri plate put it in oscar if it ʼ s

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If it ʼ s a Petri Plate - Put it in Oscar. If it ʼ s Reusable Glassware (Test Tubes, Flasks, Bottles) - Put it in the Stainless Steel Bus Pan after you ʼ ve removed any Tape or Labels. If it ʼ s a Serological Pipette - Put it (in its Wrapper) into the Plastic Bus Pan. The Specifics for Today’s Lab are: • Microtiter Pipette Tips go into the Beaker marked “Microtiter Pipette Tips” on your Bench - Please empty this into the Stainless Steel Pitcher before you leave • Curvettes go to Oscar • Petri Plates go to Oscar • Pseudomonas Sodium Benzoate Tubes go into the Stainless Steel Bus Pan - Please remove your Label from these Tubes • Please swab-down your Bench Top with Bacdown before you leave - And don’t forget to wash your Hands Remember to save all of your Plate Isolates: • Bacillus Nutrient Agar Plates go into the 04°C Boarding Zone • Skin YGC Plates go into the 04°C Boarding Zone • Enteric Nutrient Agar Plates go into the 04°C Boarding Zone • Enteric EMB Plates go into the 04°C Boarding Zone
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Lab 2 Page 4 Microtiter Pipettes When we need incredibly precise Measurements or rapidly repeated Measurements we’ll use Microtiter Pipettes. Microtiter Pipettes used in MIC 101 Lab: Name Top Button Color Setting (Maximum) Microliters P200 Yellow 2 | 0 | 0 200.0 P1000 Blue 1 | 0 | 0 1000.0 Top Button Color indicates proper Tip Size. • The Volume can be set by turning the knurled, Black Volume Adjustment Knob on the Upper Portion of the Handle (and if knurled [“ribbed”], the White Control Knob on Top of the Microtiter Pipette). • The Volume set can be read from the Volume Indicator Display on the Front of the Handle. The Volume Indicator Display is read from Top to Bottom. - All Digits on the P200 Mitcrotiter Pipettes are Black 1 | 5 | 0 indicates a Volume of 150 μ l 2 | 0 | 0 indicates a Volume of 200 μ l - The First Digit on the P1000 Microtiter Pipette is Red (and will be a 0 unless set to Maximum Volume of 1000 ml); the other Digits are Black. 0 | 1 | 5 indicates a Volume of 150 μ l 0 | 2 | 0 indicates a Volume of 200 μ l 1 | 0 | 0 indicates a Volume of 1000 μ l • The P200 Microtiter Pipettes use Yellow Tips in Red Boxes - Yellow Top Button, Yellow Tips in Red Boxes • To maintain Aseptic Technique, keep the Tip Boxes closed except when you’re putting a Tip on your Microtiter Pipette. • The P1000 Microtiter Pipettes use Large Tips in Purple Boxes - Blue Top Button, Clear Tips in Purple Boxes
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Lab 2 Page 5 How to properly use our Microtiter Pipettes The Control Knob • The Control Knob moves a precisely sized Piston up and down within an equally precisely sized Cylinder. NOTE: If the Control Knob on your Microtiter Pipette is knurled, it can also be used to set the Volume • The Control Knob can be pressed down to a First Stop and then further depressed -- using a bit more Force -- to a Second Stop - The First Stop is the Measuring Stroke • The Selected Volume can be either aspirated (“sucked-up”) or dispensed - The Second Stop is the Blow-Out Stroke • Liquid remaining in the Pipette after Delivery from the
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