Past ingestion of glucola 75g 140 199 mgdl pre

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2 hrs. past ingestion of glucola (75g): 140-199 mg/dl –pre-diabetic >200 mg/dl -diabetes List specimen types, testing interferences and common methods used to determine glucose concentration Glucose Test –Whole Blood (finger prick), Plasma (spin blood down), Urine, CSF Whole blood levels 15% lower (b/c cells present that are utilizing glucose) Spin and separate w/in 1 hr. (important) Heparinized (green tube) or sodium fluoride (gray tube) specimens – glycolysis inhibited Interferences: hemolysis, inc. biliribon –dc. Results Mehod: *Glucose Oxidase* ( D-Glucose is oxidized in the presence of glucose oxidase to yield hydrogen peroxide and D-Gluconate. Then, hydrogen peroxide, 4-APP (4-aminoantipyrine), and phenol yield water and quinoneimine dye in the presence of peroxidase. The quinoneimine dye is measured at 500 nm, and the absorbance of the sample containing the quinoneimine dye at 500 nm is proportional to the concentration of glucose in the patient sample.) or Hexokinase Oral Glucose Tolerance Testing
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CARBOHYDRATES –OBJECTIVES Not recommended; fasting-take blood sample and urine- give glucola-every hr./30 min. repeat the process; process repeated continuously; may last 5 hrs. Hemoglobin A1C –Glycosylated Hemoglobin Many performed on those already diagnosed to see how well their maintaining it over 3 mo. (120 days)(aids in monitoring long term diabetes); amnt. of glucose bound is a rough estimate of patient glucose levels; Glucose rxs and binds to amino group of hemoglobin; RBC life of 120 days; Normal range 4-6%; Specimen: EDTA whole blood; Method: HPLC; point of care methods Glucose Metabolism
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CARBOHYDRATES –OBJECTIVES
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