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Labels usually include the organism name type of agar

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Labels usually include the organism name, type of agar, date, and the plater's name orinitials. Using sterile cotton swabs, remove any visible water on the agar in the plate oraround the inner rim of the petri plate. Observe the plate and mentally divide it intothree sectors. The plate will then be turned clockwise (if you are right handed) with theagar side up. The second sector will then be at the top for streaking and then the plateis turned again so that the third sector can be streaked.
FACULTY: ENNGINEERINGTECHNOLOGYEDITION:1LABORATORY: FERMENTATIONTECH LABORATORYREVISION NO:EXPERIMENT:Sterilizationtechnique & InnoculumpreparationEFFECTIVE DATE:1/4/2018AMENDMENT DATE:b.Sterilize the Transfer Loop before Obtaining a Specimen:To streak a specimen from a culture tube, metal transfer loops are first sterilized byflaming the wire loop held in the light blue area of a Bunsen burner just above the tipof inner flame of the flame until it is red-hot. If a hot incinerator is available, the loopmay be sterilized by holding it inside the incinerator for 5 to 7 seconds. Once sterile,the loop is allowed to cool by holding it still. Do not wave it around to cool it or blow onit. When manipulating bacteria, transfer loops are usually held like a pencil. If plasticdisposable loops are being utilized, they are removed from the packaging to avoidcontamination and after being used, are discarded into an appropriate container. Anew loop is recommended for each sector of an isolation streak plate.c.Open the culture tube and collect a sample of specimen using the sterile loop:Isolation can be obtained from any of a variety of specimens. This protocol describesthe use of a mixed broth culture, where the culture contains several different bacterialspecies or strains. The specimen streaked on a plate could come in a variety of forms,such as solid samples, liquid samples, and cotton or foam swabs. Material containingpossibly infectious agents should be handled appropriately in the lab using bio safetyprocedure.Remove the test tube cap. It is recommended that the cap be kept in your right hand(the hand holding the sterile loop). Curl the little finger of your right hand around thecap to hold it or hold it between the little finger and third finger from the back. Moderntest tube caps extend over the top of the test tube, keeping the rim of the test tubesterile while the rim of the cap has not been exposed to the bacteria. The cap can alsobe placed on the disinfected table, if the test tube is held at an angle so that aircontamination does not fall down into the tube. Insert the loop into the culture tubeand remove a loopful of broth. Replace the cap of the test tube and put it back into thetest tube rack.d.Streak the Plate:The lid of the agar plate has to be opened just sufficiently enough to streak the platewith the inoculation loop. Minimize the amount of agar and the length of time the agaris exposed to the environment during the streak process.

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Term
Fall
Professor
N/A
Tags
Bacteria, Agar plate, Petri dish, Bacterial growth

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