They keep with the specimen for a long period of time

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They keep with the specimen for a long period of time while synthetic ones easily fade away. Permanence coloration is important especially for preparations that require considerable handling over period of time. Scope and limitations This study only wants to know if it is feasible that mayana extract can be used as a biological stain for plant tissues. Also, this study finds out if it can compare to the other synthetic especially iodine solution. If it reveals microscopic details that can be found in the cell of the plants. I used plant tissue specifically onion as an specimen for testing my natural biological stain. Definition of terms Biological Stain - are frequently used in biology and medicine to highlight structures in biological tissues for viewing, often with the aid of different microscopes. Stains may be used to define and examine bulk tissues (highlighting, for example, muscle fibers or connective tissue), cell populations
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(classifying different blood cells, for instance), or organelles within individual cells. It is also used to mark cells in flow cytometry, and to flag proteins or nucleic acids in gel electrophoresis.\ Mayana plant -Erect, branched, fleshy annual herb, about 1 m high. Stems, purplish and 4-angled. Leaves are blotched or colored, ovate, 5-10 cm long, with toothed margins. Flowers are purplish, numerous, in simple or branchedinflorescences, 15-30 cm long. Chapter Two Review of Related Literature
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A wide variety of synthetic and naturally occurring biological dyes are available to the microscopist for selective staining of intracellular organelles in cells and tissues. Biological stains dramatically improve specimen contrast in brightfield illumination, and have been utilized for many years in histological preparations targeted at studies in anatomy, pathology, physiology, and similar disciplines. Compare the information contained in this section on the visible light
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