Coordination of a hydrated mg 2 ion between u1 a34

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) Coordination of a hydrated Mg 2+ ion between U1, A34 and G35. Transition metal ions are expected to directly coordinate G N7 and interact with the phosphophodiester indirectly through a water molecule. ( f ) Midpoints of self-cleavage in the presence of various divalent salts were determined as described in a and summarized as a horizontal bar graph. Error bars show s.d. of 3 independent trials. © 2017 Nature America, Inc., part of Springer Nature. All rights reserved.
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1114 NATURE CHEMICAL BIOLOGY | VOL 13 | OCTOBER 2017 | ARTICLE NATURE CHEMICAL BIOLOGY DOI: 10.1038/NCHEMBIO.2459 grooves are lined with G N7 atoms that face inward toward phos- phate groups on either side of the cleavage site ( Fig. 5d ), offering a plausible explanation for strong transition metal binding to the Osa twister RNA. The function of the twister ribozyme in the host organism O. sativa is not known. Rice seedlings rapidly take up Mg 2+ from the root tips, and Mg 2+ deficiency causes leaf yellowing and other growth defects 41 . Rice also concentrates certain transition metals in its tissue, some of which are essential micronutrients 42,43 . Our results show that transient folding in micromolar metal ions is sufficient for robust activity. Its preference for transition metal ions and small size make twister a potentially attractive starting point for engi- neered ribozymes that respond to rare or toxic metals in rice and other organisms. Received 2 December 2016; accepted 18 July 2017; published online 21 August 2017 METHODS Methods, including statements of data availability and any associated accession codes and references, are available in the online version of the paper . References 1. Jimenez, R.M., Polanco, J.A. & Lupták, A. Chemistry and biology of self-cleaving ribozymes. Trends Biochem. Sci. 40 , 648–661 (2015). 2. Roth, A. et al. A widespread self-cleaving ribozyme class is revealed by bioinformatics. Nat. Chem. Biol. 10 , 56–60 (2014). 3. Weinberg, Z. et al. New classes of self-cleaving ribozymes revealed by comparative genomics analysis. Nat. Chem. Biol. 11 , 606–610 (2015). 4. Furukawa, K. et al. Bacterial riboswitches cooperatively bind Ni 2+ or Co 2+ ions and control expression of heavy metal transporters. Mol. Cell 57 , 1088–1098 (2015). 5. Price, I.R., Gaballa, A., Ding, F., Helmann, J.D. & Ke, A. Mn 2+ -sensing mechanisms of yybP-ykoY orphan riboswitches. Mol. Cell 57 , 1110–1123 (2015). 6. DeRose, V.J. Metal ion binding to catalytic RNA molecules. Curr. Opin. Struct. Biol. 13 , 317–324 (2003). 7. Liu, Y., Wilson, T.J., McPhee, S.A. & Lilley, D.M. Crystal structure and mechanistic investigation of the twister ribozyme. Nat. Chem. Biol. 10 , 739–744 (2014). 8. Ren, A. et al. In-line alignment and Mg 2+ coordination at the cleavage site of the env22 twister ribozyme. Nat. Commun. 5 , 5534 (2014). 9. Wilson, T.J., Liu, Y., Domnick, C., Kath-Schorr, S. & Lilley, D.M. The novel chemical mechanism of the twister ribozyme. J. Am. Chem. Soc. 138 , 6151–6162 (2016).
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  • Spring '13
  • Nelson
  • RNA, Wind, Ribozyme, Hammerhead Ribozyme, Osa twister

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