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MIC 101 Exam 1 Winter 2013

Louis pasteur was aware that the causative agent of

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19. Louis Pasteur was aware that the Causative Agent of Rabies was a bit unusual. How did Pasteur make a Rabies Vaccine? A. The Spore-Forming, Gram Negative Bacterium was attenuated by growing it at 42°C, at which Temperature it can no longer form Endospores. B. The Spore-Forming, Gram Positive Bacterium was attenuated by infecting an alternate Host (Rabbits) and then desiccating the infected Brains and Spinal Cords C. The Negative Sense RNA Virus (Baltimore Class V) was attenuated by infecting an alternate Host (Rabbits) and then desiccating the infected Brains and Spinal Cords D. The Double-Stranded DNA Virus (Baltimore Class I) was attenuated by growing it at 42°C, at which Temperature it can no longer infect Nervous Tissue. STOP! Double-Check your ScanTron or be Stupid. You have just answered Question 19. Proceed to Question 20.
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MIC 101 Exam 1 Page 12 20. Our Evil Scientist asks her Lab Assistant Carl -- a former East German Olympic Gold Medal Swimmer -- to make two different Batches of Media and to use these Media to simultaneously isolate both Aerobic and Microaerophilic Bacteria. “The last Lab Assistant who tried to simultaneously isolate Aerobic and Microaerophilic Bacteria for me did not succeed. So I keyed his brand new shiny Plasma Blue Pearl Subaru WRX STI Limited. I ʼ ve noticed your brand new shiny Black Sapphire Metallic BMW 335i Coupe in the Parking Lot. You wouldn ʼ t want anything to happen to it, would you?” No, Carl does not want anything to happen to his brand new shiny Black Sapphire Metallic BMW 335i Coupe. Definitely not. He does not enjoy hearing any of Our Evil Scientist ʼ s horrific and all too plausible Scenarios. “Then hop to it, Speedo!” Quick like a Bunny Carl goes to the Lab Computer to look-up the Recipes for Our Evil Scientist ʼ s Media (ESM-1 and ESM-2). The Recipes are: ESM-1 ESM-2 1 Liter Distilled Water 1 Liter Distilled Water 3 Grams Beef Extract 10 Grams Yeast Extract 5 Grams Sodium Chloride 20 Grams Calcium Carbonate 5 Grams Peptone 20 Grams Glucose 15 Grams Agar 20 Grams Agar These Recipes tell you that -- A. • ESM-1 and ESM-2 are both Defined (Synthetic) Media • Carl will use Spread Plate Technique. B. • ESM-1 and ESM-2 are both Undefined (Complex or Enriched) Media • Carl will use Pour Plate Technique. C. • ESM-1 is a Defined (Synthetic) Medium • ESM-2 is an Undefined (Complex or Enriched) Medium • Carl will use Pour Plate Technique. D. • ESM-1 is an Undefined (Complex or Enriched) Medium • ESM-2 is a Defined (Synthetic) Medium • Carl will use Spread Plate Technique.
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MIC 101 Exam 1 Page 13
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