Module_07_Lab_Worksheet_05202018.docx

The anatomy and physiology associated with the

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the anatomy and physiology associated with the drawing of the frontal plane view of a kidney and the other group do the same for the drawing of the nephron. Part 02 Procedure: Urinalysis 1. Perform a mid-stream sterile urinalysis specimen on yourself with a sterile urine collection cup 2. Using universal precautions, perform a physical inspection and urinalysis test on your own urine specimen and two other urine specimens. Record the
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results of your observations and test results in the chart below: 3. Record the results of the urinalysis in the chart below. Test Self Specimen Classmate Specimen A Specimen B Color Yellow Yellow Yellow Yellow Odor Ammonia/swe et None Ammonia Ammonia Turbidity Slightly hazy Clear Clear Slightly cloudy Glucose Negative Negative 0.250 Negative Bilirubin 17 Negative Normal 1 Negative Ketone 1.5 Negative Negative 0.5 Specific Gravity 1.15 1.015 1.005 1.010 Hemoglobin Minimal/ trace Negative Negative Negative pH 6.5 6.5 5.0 6.0 Protein 0.15 Negative Negative Negative Urobilinogen Normal 1 Normal 1 Normal 1 Negative Nitrite Negative Negative Negative Negative Leukocytes Negative Negative Negative Negative 4. Part 03 Procedure: Microscopic Urinalysis 1. With your urine sample, pipette your urine into two urine centrifuge tubes if you have enough urine specimen. If not, one sample will be proficient. Make sure both tubes have the same amount of urine within them. 2. Hand your specimens to your instructor to be centrifuged. When the centrifuged process is completed, place the urine centrifuge tubes in a beaker and carefully return to your workstation. You don’t want to jostle or bump the centrifuged tubes. 3. At your workstation, carefully examine the centrifuged tubes. You should notice a small layer of sediment. You will CAREFULLY decant the supernatant so just the sediment is left- basically pour off the urine so that the sediment is just left; you may have to pipette the last bit of the urine. Again, you do not to
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disturb the sediment during this process. 4. Once the supernatant is decanted and only the sediment is left, re-suspend the sediment in the remaining fluid by flicking the bottom of the test tube a few times. Once the sediment is re-suspended, pipette the sediment and place a drop on microscope slide and place a coverslip over it. 5. Microscopically examine the sediment of the urine sample. You may have to adjust the condenser of the microscope and the amount of light with the iris to have maximum viewing capabilities. Please note your observations of the various cells and substances that can be observed that include cellular structures, casts, foreign objects, crystals and pathogens: Erythrocytes : few present scattered throughout slide; they appear medium in size (smaller than leukocytes), spherical shaped cells translucent middle Leukocytes : Not present; however, they appear largest in size, spherical shaped cells (Betts, 2017).
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