Making three germ layers Figure 17 The dividing cells of the fertilized egg

Making three germ layers figure 17 the dividing cells

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--- Making three germ layers
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Figure 1.7 The dividing cells of the fertilized egg form three distinct embryonic germ layers Three germ layers --- first step in differentiation
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Figure 1.11 Fate maps of vertebrates at the early gastrula stage Cell fates: developmental destinations of a given cell --- differentiation Blastula/Gastrula stage Dorsal View How can we make a fate map?
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Figure 1.12 The fates of individual cells --- Tunicates
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Figure 1.13 Vital dye staining of amphibian embryos Marking cells to trace cell lineages
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Figure 1.14 Fate mapping using a fluorescent dye Injection of Fluorescein-conjugated dextran into individual cells
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Figure 1.15 Genetic markers as cell lineage tracers Antibodies against a protein unique to quail Quail cell has a large nucleolus. Can we do a chick-to-chick transplantation experiment? Limited to cells with pigments Is there any solution to this problem?
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Figure 1.16 Fate mapping with transgenic DNA shows that the neural crest is critical in making the gut neurons How can we make a chick green?
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Reporter genes GFP (Green Fluorescent Protein) lacZ (bacterial gene, b -galactosidase ) GFP TATA Chimeric gene (artificial gene)
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Chimeric gene (artificial gene) --- Transgene What gene’s enhancer allows us to express GFP in all the cells in the body? GFP TATA Housekeeping genes such as actin Actin gene’s enhancer GFP TATA
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