000025 wv malachite green 15 wv agar pH 66 SteriHse at 121C for 15min cool to

000025 wv malachite green 15 wv agar ph 66 sterihse

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0.000025% (w/v) malachite green 1.5% (w/v) agar pH 6.6 SteriHse at 121°C for 15min, cool to 50-55°C and add supplements of either bovine albumin—fraction V, glucose and beef catalase (to 0.5% w/v, 0.2% w/v and 0.0003% w/v, respectively); oleic acid, bovine albumin—fraction V, glucose , beef catalase and sodium chloride (to 0.005% v/v, 0.05% w/v, 0.2% w/v, 0.0004% w/v and 0.085% w/v, respectively); or oleic acid, bovine albumin—fraction V, glucose, beef catalase, sodium chloride and Triton (to 0.005% v/v, 0.05% w/v, 0.02% w/v, 0.0004% w/v, 0.085% w/v and 0.025% v/v, respectively). Myxobacterium selective medium (Hsu et al, 1983) 0.3% (w/v) casein 0.2% (w/v) tryptone 0.05% (w/v) yeast extract 0.03% (w/v) calcium chloride 1.0% (w/v) agar pH 7.0 SteriHse at 12rC/15min, allow to cool to ~50°C, and add filter-sterilised anti- biotic solution: 10|ig/ml of erythromycin, 10|ig/ml of neomycin sulphate or 256TU/ml of polymyxin sulphate (this may be replaced by colistin sulphate). Peptone beef extract glycogen agar (PBG) 1% (w/v) beef extract 0.5% (w/v) glucose 1% (w/v) peptone 0.5% (w/v) sodium chloride 0.004% (w/v) bromothymol blue 1.5% (w/v) agar 2% (w/v) agar (for overlay)
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Isolation/Detection 179 Sterilise separately at 121°C/15min. The basal medium is used for pour plates, after which the water agar is used as an overlay. Petragnani medium 900 ml 36 g 500 g 1,200 ml 70 ml l-2g pH whole milk potato flour potato whole egg (whites and yolks) glycerol malachite green 7.2 Photobactevium damselae subsp. piscicida medium (Hashimoto et ciL, 1989) 1% (w/v) casamino acids/polypeptone 0.5% (w/v) yeast extract 0.2% (w/v) galactose 1 % (w/v) sodium glutamate 0.5% (w/v) magnesium acetate Agar may be added to solidify the medium. Pseudomonas F agar 1% (w/v) tryptone 1 % (w/v) proteose peptone 0.15% (w/v) dipotassium phosphate 0.15% (w/v) magnesium sulphate 1.% (w/v) agar 1% (v/v) glycerol pH 7.0 Sterilise at 121°C/15min. Ribose ornithine deoxycholate medium for the isolation of Yersinia ruckevi (Rodgers, 1992) 0.3% (w/v) yeast extract 0.1% (w/v) sodium deoxycholate 0.5% (w/v) sodium chloride 0.68% (w/v) sodium thiosulphate 0.08% (w/v) ferric ammonium citrate 0.75% (w/v) maltose 0.375% (w/v) ribose 0.375% (w/v) ornithine hydrochloride 0.1% (w/v) sodium dodecyl sulphate 0.008% (w/v) phenol red 1.25% (w/v) agar pH 7.4 10 ml of a filtered (0.22 |im) solution containing 0.5 g sucrose/ml should be added after the basal medium has been autoclaved (12rc/15min) and cooled to 50°C.
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180 Bacterial Fish Pathogens Rimler-Shotts medium (Shotts and Rimler, 1973) 0.05% (w/v) 0.65% (w/v) 0.35% (w/v) 0.68% (w/v) 0.03% (w/v) 0.003% (w/v) 0.08% (w/v) 0.1% (w/v) 0.0005% (w/v) 0.3% (w/v) 0.5% (w/v) 1.35% (w/v) pH L-lysine hydrochloride L-ornithine hydrochloride maltose sodium thiosulphate L-cysteine hydrochloride bromothymol blue ferric ammonium citrate sodium deoxycholate novobiocin yeast extract sodium chloride agar 7.0 After boiling to dissolve the ingredients, the medium is not sterilised further. Robertsons meat broth (= cooked meat medium) 45.4% (w/v) heart muscle 1% (w/v) peptone 1% (w/v) Lab-lemco powder 0.2% 9w/v) dextrose 0.5% (w/v) sodium chloride pH 7.2 SteriHseat 12rc/15 min.
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