Biology_2007_Virtual_Lab_Answer_Key-1

3 what other problems might dna fingerprinting of

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3. What other problems might DNA fingerprinting of bear hair solve? DNA fingerprinting on bears might help gather data to determine which pairs of bears are producing offspring. It will give data on the breeding patterns of the bear population. 4. The police arrested three suspects and obtained DNA fingerprints from each individual. What does the investigator need to establish to determine which of the three might be the culprit in the crime? Explain your answer. A DNA sample from the crime scene is needed to make a DNA fingerprint so that the suspect’s DNA fingerprints can be compared to the sample. 5. How could DNA fingerprinting technology is used to establish that a highway cutting through grizzly bear habitat stops the bears from moving from one side to the other? Explain your answer. DNA fingerprinting could establish breeding patterns and show that the bears on one side of the highway are not related to the bears on the other side, and that there are two distinct bear populations that are not interbreeding.
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6. Monarch butterfly populations are found east and west of the Rocky Mountains. How could DNA fingerprinting technology be used to establish that they constitute one breeding population or two separate breeding populations? Explain your answer. If the genetic fingerprints of the two populations were sufficiently different, this would provide support for the hypothesis that they do not interbreed. If they are similar the hypothesis, that the two populations are one breeding population, is supported. 7. Describe the advantages and disadvantages of radiotelemetry and DNA fingerprinting methods for obtaining wildlife population data. Radiotelemetry has the advantage of providing data on the health, age, and reproductive state of the captured and sedated animals. Radiotelemetry has the disadvantage of being very intrusive into the wildlife from which data are to be collected. Fewer animals can be sampled than with DNA fingerprinting methods from hair or scat samples. Genetic methods are less intrusive but will not give information on the age, health and reproductive state of the animals. Lab 15: Gene Splicing Analysis The answers are intended only as samples. Answers also depend on how students completed the lab and on the random data generated by the computer. 1. DNAis made up of two separate strands of base sequences. The same sequence is found on both strands, but running in opposite directions. What word describes this characteristic? Palindrome. Palindromes are words or sentences that read the same forwards or backwards, such as ‘Mom’ or ‘Dad’. 2. What does the term ‘sticky ends’ refer to in gene splicing? Some restrictive enzymes cut DNA sequences so that double-stranded fragments with single-stranded ends are formed. The dangling single stranded ends have a tendency to join with other single-stranded ends to become double-stranded, so they tend to attract DNA that they can join with. For this reason, these ends are called sticky ends.
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