2 2csts are made of sensor kinases and response

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(2) 2CSTS are made of sensor kinases and response regulators (2) The Sensor kinase consists of 2 domains (Sensor and kinase), and is often located in the cytoplasmic membrane. (2) The sensor domain is more variable and detects/binds environmental signal (1), (2) After receiving signal, the kinase domain phosphorylates itself on a conserved Histidine in its conserved kinase domain. (2) The P-Sensor Kinase contacts the conserved reciever domain of the Response Regulator which is cytoplasmic (2) The phosphate group is transferred to the conserved Aspartate in the conserved reciever domain. (2) The effector domain (or DNA binding) domain, which is less conserved, of the R.R. will bind to DNA and activate transcription when the N-terminal domain is phosphorylated. 12 69 16 lysogenic bacteriophage 12 116 17 RssB and phosphorylated 12 80 18 Sigma 54 and Sigma 70 12 112 19 Microarray 11 49 20 intermediate 12 111 21 I and alpha 12 45 22 ATP binding/hydrolysis OR interaction with RNAP- σ 54
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Microbial Genetics BIOL518 Exam 4 - Fall 2010 Name_____________________________________________________________________ Multiple Choice. Choos e all of the correct answers for each question. There may be more than one correct answer per question. (52 points, 4 pts each) 1: The glutamine synthetase enzyme, encoded by the glnA gene: A: catalyzes the reaction: (ammonia) NH 3 + glutamate ---> glutamine. B: is synthesized at a higher rate by sigma 54/N under low NH 3 conditions. C: is responsible for the vast majority of ammonia assimilation under low ammonia conditions. D: is encoded in an operon that also encodes the regulatory proteins of the operon. 2: When there is essentially no free tryptophan in an E. coli cell, but the majority of the appropriate tRNA is charged with tryptophan: A: A ribosome translating the trpEDCBA operon leader peptide will stall at the stop codon. B: A stalled ribosome will cover regions 1 and 2 of the trpEDCBA operon leader region. C: Regions 3 and 4 of the trpEDCBA operon leader will form a stem loop. D: Transcription of the trpEDCBA operon will terminate before it reaches trpE . 3: Within the phosphoenolpyruvate (PEP) dependent sugar phosphotransferase system (PTS) A: Glucose is phosphorylated (to Glucose-6-phosphate) by phosphorylated IIA Glc B: Unphosphorylated IIA Glc will activate adenylate cyclase C: Unphosphorylated IIA Glc will inhibit transport of lactose (LacY) D: Low glucose levels will almost immediately result in high concentrations of unphosphorylated IIA Glc 4: During the stationary phase of bacterial growth A: expression of genes that encode virulence factors can be induced B: the associated transcriptional response is almost identical to that of bacteria grown in the absence of glucose but the presence of galactose C: the transcriptional response is also called a general stress response D: the master regulator of the transcriptional response observed is an alternative sigma factor termed Sigma S (RpoS) 5: During the classical heat shock response (temperature increase), A: proteins become toxic to a bacterium due to the extreme stability and maintence of normal activity B: genes encoding proteases are dramatically upregulated and chaperones are downregulated C:
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