Part b after completing the steps described above you

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Part B. After completing the steps described above, you also must perform an experiment to determine the value of n , the order of reaction of Blue#1 with respect to [OCl - ]. If in doubt, talk with your TAs. Part C. After completion of the experiments described above, you must study the kinetics of the reaction of Green food dye with OCl . You should begin, of course, by
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Chemistry 132 Lab Manual Page 59 recording the spectrum of an appropriate concentration of a Green food dye solution, printing the spectrum for your lab report, and recording the absorbance maxima for the Green food dye solution. For the Green food dye you must monitor the reaction at two wavelengths, simultaneously, 630 nm and 440 nm, which is a ‘shoulder’ on the low- wavelength absorption band. Explain in your lab notebook, and in your lab report, why these two wavelengths are selected. Directions for collecting data at two wavelengths are given below. You should record in your notebook, and include in your lab report, any visual changes observed for the reaction of Green food dye with OCl and compare these to the reaction of Blue food dye (Blue#1) with OCl . Part D. Determine the rate constant for reaction of Yellow food dye with OCl . We hope you enjoy designing experiments for this reaction. Remember that TAs are available to provide help when needed. Before you begin you should consider your observations and data for the blue and green food dyes, to provide guidance on how to adapt conditions for studying the yellow food dye. Using the Red Tide 650 Spectrophotometer for Kinetics Experiments After you have recorded a spectrum of Blue#1 and determined the wavelength you wish to monitor, have one partner prepare the sample for your kinetic run while the other partner sets up the Red Tide 650 spectrophotometer. Directions for collecting data at one wavelength. See instructions on the following page for collecting data at two wavelengths. 1. Under “File” choose “New” and then “Strip Chart Table” at the bottom of the menu. 2. A new tab will appear and a “Chart Trend Settings” dialog box will open on the screen. On the left side of the “Chart Trend Settings” dialog box, click the radio button for “Update with the first available scan every:”. Set the time for 10 seconds. click the box for “Stop after this amount of time:”. Set the time for 6 minutes for your first kinetic run. On the right side of this dialog box if not already selected, click the radio button for “Single Trends from This Source”
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Chemistry 132 Lab Manual Page 60 Set the wavelength to the value desired DO NOT CLICK THE “ACCEPT” BUTTON IN THE “Chart Trend Settings” DIALOG BOX until your sample is in the spectrophotometer. 3. Immediately after you have mixed the reagents and filled the cuvette about 2/3 full, wipe the cuvette with a Kimwipe, and place the cuvette in the Red Tide spectrophotometer, and click the “Accept” button in the Chart Trend Settings dialog box to start your experiment.
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