Case Notes - DNA Analysis Results.docx

The three evidence items 23b2b blood spatter from

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the chelex method. The three evidence items, 23(b2b) – blood spatter from Richard’s left shoe, 45(d1b) – blood spatter from Ralph’s shoes, and 54(e) – blood spatter from the crowbar, were all extracted using the organic method. All five samples were then quantified and amplified using a GeneAmp PCR System 9700 thermal cycler. The samples were then analyzed on a 3130xl Genetic Analyzer capillary electrophoresis. DNA profiles were interpreted using SoftGenetics GeneMarker HID V2.7.1 software. Statistical data for the profiles was determined using the FBI Stats Sheet. The resulting electropherograms for the five evidence items above were interpreted as follows: 1 FRNSC 100
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Initials: MM Date: 07-APR-2018 P ENN S TATE U NIVERSITY C RIME L AB 1313 Shaler Way University Park, PA 16802 3(a) Reference Profile 23(b2b) Unknown Evidence 42(a1) Reference Profile 45(d1b) Unknown Evidence 54(e) Unknown Evidence LOCUS D8S1179 10,15 10,15 14,14 10,15 10,15 D21S11 30,31 30,31 30,30 30,31 30,31 D7S820 10,11 10,11 8,9 10,11 10,11 CSF1PO 11,12 11,12 11,13 11,12 11,12 D3S1358 17,18 17,18 15,15 17,18 17,18 TH01 6,9 6,9 9.3,9.3 6,9 6,9 D13S317 12,14 12,14 9,12 12,14 12,14 D16S539 9,12 9,12 11,12 9,12 9,12 D2S1338 19,20 19,20 17,24 19,20 19,20 D19S433 12,15 12,15 12,14 12,15 12,15 vWA 15,19 15,19 15,16 15,19 15,19 TPOX 8,8 8,8 8,11 8,8 8,8 D18S51 12,14 12,14 14,17 12,14 12,14 Amelogenin X,Y X,Y X,Y X,Y X,Y D5S818 11,13 11,13 10,11 11,13 11,13 FGA 24,25 24,25 22,24 24,25 24,25 Conclusions: Item 42(a1) – Ralph P. reference profile: 2 FRNSC 100
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Initials: MM Date: 07-APR-2018 P ENN S TATE U NIVERSITY C RIME L AB 1313 Shaler Way University Park, PA 16802 The likelihood that this profile would randomly occur in the world population, according to the FBI Stats Table, is as follows: Population Likelihood CAU 1 in 2.7 QUADRILLION AA 1 in 55.0 QUADRILLION
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  • Fall '18
  • World population, Pennsylvania State University, DNA profiling, University Park, Pennsylvania, Ralph P.

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