L.02. Growth of Bacteria

We use e coli strain ml 30 in lab alice audureau a

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We use E. coli Strain ML-30 in Lab. Alice Audureau, a French Graduate Student at The Institute Pasteur in Occupied Paris, isolated this Strain from Microbiologist Andre Lwoff in the 1944. The “ML” stands for “Mutabile in Lwoffi” (an Institute Pasteur “inside” Joke). - Strain O157:H7 • 5,594,477 (5.6 Mbp / 5590 kbp) The larger Genome Size is due to the Presence of as many as 15 different Pathogenicity Islands. - Approximately 300 different Plasmids have been isolated from E. coli • Among the Bacteriophage (Bacterial Viruses) that attack E. coli are -- - T Phage (T= Type), λ (Lambda), μ (Mu), φ X174 (Phi X 174) We use φ X174 for our Bacteriophage Titer Lab. • Restriction-Modification Enzymes - Eco RI Restriction Endonuclease 5’XXXGAATTCXXX3’ 5’XXXG- -AATTCXXX3’ 3’XXXCTTAAGXXX5’ 3’XXXCTTAA- -GXXX5’ Restriction Enzymes recognize and destroy Foreign DNA (i.e. Bacteriophage DNA). Cleavage of DNA with Eco RI results in “Sticky Ends”. - Eco RI Methylase “Modifies” (methylates/adds a CH 3 Group) the most internal Adenines 5’XXXGAATTCXXX3’ 3’XXXCTTAAGXXX5’ Modification of even one of the Adenines in its own Sequences allows E. coli to be immune to the Action of its Restriction Enzyme Eco RI.
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Growth of Bacteria Page 3 Why is Escherichia coli (E. coli ) the best-known Organism on this Planet? Like the Question as to why Mice became the Animal Model of Choice for Immunologists (and do not get me started on this), it ʼ s partly a Matter of Luck and partly a Matter of Choice. - By the Late 1930s E. coli had become a popular Bacterium because was easy to obtain and easy to grow in Culture (Choice). You can literally pull it out of your Butt. - In 1939 The Phage Group (a Group of disenchanted and/or refugee Physicists) decided to try and elucidate “The Secret of Life” using the T-Even Bacteriophage, which happen to grow in E. coli (Luck). - In 1942 Salvador Luria and Max Delbrück ʼ s “Fluctuation Test” demonstrated that Mendelian Genetics applied to E. coli (Choice). It may seem incredibly now but up until 1942 there was considerable Debate as to whether the Laws of Mendelian Genetics applied to Bacteria.
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  • Winter '09
  • MANN
  • Bacteria, Escherichia coli

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