Tissue specimen for microscopic analysis involves

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Introduction to Health Care
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Chapter 1 / Exercise 3
Introduction to Health Care
Haroun/Mitchell
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tissue specimen for microscopic analysis involves many steps and is complicated but essentially involves dehydration, clearing, bathing in melted paraffin to refill the tissue spaces, cooling, then placing in a block of paraffin which is sliced with a microtome in section 0.005 mm thick. The sections are, in essence, glued to a slide and then stained, and covered with another slide for permanent sections. 6. Define the following terms: mordant, decolorizer, counterstain, polychrome stain, metachromatic stain. A mordant is a substance that renders the tissue chemically more receptive to staining. A decolorizer removes the initial stain from all parts of the tissue to which it has not become chemically bound. A counterstain is a contrasting color taken up by tissues that have been decolorized after the application of the first stain. A polychrome stain is a mixture of two or more coloring agents in one solution whereby differential staining of tissue components takes place even though the tissue is exposed to all of the coloring agents simultaneously. A metachromatic stain is one that changes color on becoming chemically bound to certain tissues. 7. What is the most common stain used in preparing specimens for histologic examination? H&E (hematoxylin and eosin). 120
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Introduction to Health Care
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Chapter 1 / Exercise 3
Introduction to Health Care
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CHAPTER 19 PATHOLOGY EXERCISES AT END OF CHAPTER Medical Vocabulary Review 1. A microscopic connective tissue layer underlying an epithelial surface and also investing certain kinds of cell is _____ D. the basement membrane. 2. Dysfunction of the sphincter of Oddi, the sphincter between the liver and pancreas is _____ A. biliary dyskinesia. 3. An entire specimen sample consisting of multiple smaller pieces of tissue is called ______ C. aggregate. 4. The terms portal and hepatic both refer to the ______ B. liver. 5. Thin-walled terminal capillaries having little or no adventitia and found in the liver, adrenals, heart, parathyroid, parotid gland, spleen, and pancreas are called ______ C. sinusoids. 6. A benign tumor arising from glandular epithelium is _____ B. an adenoma. 7. Highly anaplastic (undifferentiated) forms of certain malignant neoplasms containing relatively few cells might be said to be ______ D. paucicellular. 8. Tissue that is crumbly; fragmenting or bleeding easily on touch or manipulation is said to be ______ B. friable. 9. Grape-shaped secretory units in a gland are _____ A. acini. 10. The physiologically active tissue of an organ, as opposed to fat and connective tissue is the ______ C. parenchyma. 11. ______ is another name for the connective tissue sheath of the liver. B. Glisson capsule 12. Black coloration of lung tissue of little or no pathologic significance caused by inhalation of tiny particles of carbon, seen in smokers and those who live in industrial or urban environments might be referred to as _____ D. anthracotic pigmentation. 13. A semisolid film on a wound or ulcer consisting largely of the insoluble protein fibrin is _____ A. fibrinous exudate.

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