Protein Speed up chemical reactions Lower activation energy barriers Catalyst A

Protein speed up chemical reactions lower activation

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Protein Speed up chemical reactions Lower activation energy barriers Catalyst : A substance that causes a chemical reaction without itself being affected Very precise in structure Lock and key model K. BARRETTO FOR BIOL101 | LAB ACTIVITIES FROM DOWDY & YONENAKA 2018
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“LOCK AND KEY” MODEL K. BARRETTO FOR BIOL101 | LAB ACTIVITIES FROM DOWDY & YONENAKA 2018
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Substrate : Molecule to be acted on by an enzyme (hydrogen peroxide) Active Site : Spot on enzyme where substrate attaches Products : Molecules that result from the enzyme acting on the substrate (water, oxygen) K. BARRETTO FOR BIOL101 | LAB ACTIVITIES FROM DOWDY & YONENAKA 2018
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e.g. H 2 O 2 e.g. H 2 O + O 2 You save a lot of energy with enzymes! K. BARRETTO FOR BIOL101 | LAB ACTIVITIES FROM DOWDY & YONENAKA 2018
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RATE OF CELLULAR PROCESSES A lot of stuff happens in your cells! All of these reactions have to be done at a speed that sustains life Enzymes make this possible by reducing activation energy, bringing molecules together, and speeding up reactions K. BARRETTO FOR BIOL101 | LAB ACTIVITIES FROM DOWDY & YONENAKA 2018
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DIGESTION Last week: Breaking big pieces of food into small, individual molecules Enzymes are involved in this process! K. BARRETTO FOR BIOL101 | LAB ACTIVITIES FROM DOWDY & YONENAKA 2018 Lactase
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LACTASE BREAKS LACTOSE INTO SMALLER SUGARS K. BARRETTO FOR BIOL101 | LAB ACTIVITIES FROM DOWDY & YONENAKA 2018
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LACTOSE INTOLERANCE HAPPENS WHEN YOU DON’T MAKE LACTASE K. BARRETTO FOR BIOL101 | LAB ACTIVITIES FROM DOWDY & YONENAKA 2018
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K. BARRETTO FOR BIOL101 | LAB ACTIVITIES FROM DOWDY & YONENAKA 2018
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PROCEDURE 1. Identify volunteers 2. Blind taste-test: Volunteers will try Sample 1 and Sample 2 3. We will share their observations with the class. ( Note: Does one taste sweeter than the other? ) K. BARRETTO FOR BIOL101 | LAB ACTIVITIES FROM DOWDY & YONENAKA 2018 Substance Observations/ Notes Hypothesis for why it tasted the way it did (based on enzymes) Untreated Milk Pre-Treated Milk
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PROCEDURE K. BARRETTO FOR BIOL101 | LAB ACTIVITIES FROM DOWDY & YONENAKA 2018 4. Make a prediction for why one might taste sweeter than the other based on enzyme activity. 4. Make a prediction for sweeter than the other based on enzyme activity. 5. Discuss as class 5. Discuss as class
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CATALASE CASE STUDY Activity 2 K. BARRETTO FOR BIOL101 | LAB ACTIVITIES FROM DOWDY & YONENAKA 2018
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WHERE ARE ENZYMES FOUND?
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  • Spring '14
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  • Enzyme, Tendamistat

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