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Active and unhindered enzymes photometric

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active and unhindered Enzymes Photometric, fluorometric, chemiluminescence; enzymes are biological catalysts that increase the rate of conversion of substrate to product and are not consumed in the rx, as such, an enzyme can catalyze many substrate molecules, amplifying the amnt of product generated, the enzyme activity may be monitored directly or by measuring the product formed or by measuring the effect of the product on a coupled rx, depending on the substrate used the product can be photometric, fluorometric, of chemiluminescent. Fluorescent Fluorochromes or fluorophores; compounds that absorb radiant energy of one wavelength and emit radiant energy of a longer wavelength Luminescent Emit photons of light as result of electrical, biochemical or chemical reaction Describe the 3 Methods Used to Separate Unbound-Labeled (Fee) Reagent from Bound-Labeled Reagent Adsorption Uses particles such as charcoal and dextran to trap small Ag Precipitation “salt out” Abs –using ammonia sulfate, sodium sulfate Interaction with Solid Phase Ab-Ag complexes linked to solid phase support ( polystyrene, magnetic beads) –unbound not linked
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