4 incubate your sodium benzoate plate at 30c for 48

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4. Incubate your Sodium Benzoate Plate at 30°C for 48 Hours. 5. Remove the Tape from your Test Tube and place it in the Stainless Steel Bus Pan. Bacillus (Selection) The Genus Bacillus is like the Genus Canis in that saying you ʼ ve got a Bacillus is like saying you ʼ ve got a Dog. There are Tons of different Bacillus Species with different Morphologies and you may well have several different Colony Morphologies on your Plate. The Flat, Frosted Glass/Matte Colonies are the Safest Bet. Avoid Colonies that have a spreading swirling Texture. These are almost certainly Bacillus mycoides and given Half a Chance it ʼ ll overgrow every other Bacillus in your Isolate. 1. Check the Nutrient Agar Plate that you streaked with the Pasteurized Soil Suspension for Colonies that are Flat, Irregular, and have a Frosted Glass or Construction Paper Texture (“rough” or “dry” Appearance). These are likely Candidates for Bacillus . 2. Use your Sterilized Loop to gather Bacteria from a Bacillus Colony and streak this Bacillus onto a (labeled!) Nutrient Agar Plate. Feel free to use several different Bacillus Colonies to streak several Nutrient Agar Plates. 3. Incubate your new Bacillus Plate(s) at 30°C for 48 Hours. 4. Save your initial Bacillus Plate at 04°C.
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Lab 2 Page 14 Skin Bacteria (Selection) 1. Check your YGC Plate for Yellow Colonies not surrounded by a Zone of Clearing or White-to-Cream Colonies surrounded by a Zone of Clearing. • Yellow Colonies not surrounded by a Zone of Clearing are probably Micrococcus sp. • White Colonies surrounded by a Zone of Clearing are probably Staphylococcus epidermidis 2. Use your Sterile Loop to gather Bacteria from the Edge of a White or a Yellow Colony surrounded by a Zone of Clearing, and streak a (labeled!) YGC Plate. 3. Incubate your YGC Plate(s) at 37°C for 48 Hours. 4. Save your initial Staphylococcus YGC Plate at 04°C. Enteric Bacteria (Selection) Do not streak or make Smears from your EMB Plate. Your EMB Plate is for Identification Purposes only. The Enterics growing on this Plate are very Unhappy Bacteria and since the Medium contains the Dyes Eosin and Methylene Blue the Bacteria are already stained. 1 Check your EMB Plates for Purple Colonies surrounded by a Fly-Butt Green Metallic Sheen (E. coli ) or Hot Pink Colonies (Enterbacter ). This EMB Plate is only used to identify your Enteric Isolate. 2. Check your Nutrient Agar Plate for White-to-Clear Circular Convex Colonies. 3. Use your Sterilized Loop to gather Bacteria from an Enteric Colony on your Nutrient Agar Plate and streak this Enteric onto a (labeled!) Nutrient Agar Plate. 4. Incubate your new Enteric Nutrient Agar Plate at 37°C for 24 Hours. 5. Save your initial Enteric EMB and Nutrient Agar Plates at 04°C. ___________________ “I don't know. I feel like... “ Feel like what?’ “Like we're being watched.” Away put your weapon. I mean you no harm.’ © 1980 LucasFilm Ltd.
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Lab 2 Page 15 Making a Smear (Goldilocks Method) The Ability to make a Good Smear is a Make-or-Break Proposition in Lab but it ʼ s easily learned. One Lab Partner should use your Patient Skin Swab Bacterium; the other should use your Patient Rectal Swab Bacterium.
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