3 use your transfer loop to pick up a very small

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3. Use your Transfer Loop to pick-up a very small Amount of Bacteria from the Edge of a well-isolated Colony of your Enteric Isolate. 4. Transfer these Bacteria to a Labeled Tube of MR-VP Broth. Again, up and down at the Air-Liquid Interface. 5. Resterilize your Loop. 6. Place your labeled and inoculated MR-VP Broth Tube into a Plastic Cup and incubate it at 37°C for 48 Hours. Citrate 1. Sterilize your Loop in the Flame of your Bunsen Burner. 2. Cool your Sterilized Inoculating Loop on a Portion of your Enteric Isolate Plate (either Enterobacter or E. coli ) without any Colonies. 3. Use your Transfer Loop to pick-up a very small Amount of Bacteria from the Edge of a well-isolated Colony of your Enteric Isolate. 4. Transfer these Bacteria to a Slant of Citrate by using the Loop to slalom up the Surface of the Slant. The Citrate Test is Aerobic so there ʼ s no need to be Samurai Microbiologist. 5. Resterilize your Loop. 6. Place your labeled and inoculated Citrate Slant in a Plastic Cup and incubate it at 37°C for 48 Hours. _____________ “What is Sankara?” Fortune and glory, kid. Fortune and glory.’ © 1984 LucasFilm, Ltd.
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Lab 6 Page 17 Isolations Pseudomonas 1. Look for Clear-to-White Colonies. 2. Make a Gram Stain and confirm the Presence of Gram Negative Rods. 3. You can use your Eyepiece Reticle to measure the Length and Diameter of your Gram Negative Rods. Record these Numbers in your Lab Notebook. These Numbers should match your Measurements from last Week. 4. Inoculate your Pseudomonas Isolate onto F Agar (Brown Stripe) using the same Colony you used to make your Gram Stain (4 F Agar Plates per Clan). 5. Incubate at 30°C for 48 Hours. 6. Save your Pseudomonas Nutrient Agar Plate at 04°C. Bacillus sp. 1. Look for White Colonies with a Frosted Glass Appearance. 2. Make a Gram Stain and confirm the Presence of Gram Positive Rods. 3. You can use your Eyepiece Reticle to measure the Length and Diameter of your Gram Positive Rods. Record these Numbers in your Lab Notebook. These Numbers should match your Measurements from last Week. 4. Save your Bacillus Nutrient Agar Plate at 04°C. Staphylococcus 1. Look for Yellow Colonies not surrounded by a Zone of Clearing or White Colonies that are surrounded by a Zone of Clearing. 2. Make a Gram Stain and confirm the Presence of Gram Positive Cocci. 3. You can use your Eyepiece Reticle to measure the Diameter of your Gram Positive Cocci. Record these Number in your Lab Notebook. These Numbers should match your Measurements from last Week. 4. Inoculate your Skin Isolate onto YGC Agar (Red Stripe) using the same Colony you used to make your Gram Stain (1 YGC Plate per Clan). 5. Incubate at 37°C for 48 Hours. 6. Save one (1) Staphylococcus Mother Plate at 04°C.
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Lab 6 Page 18 Enteric Bacteria 1. Check your Enterobacter aerogenes or E. coli Nutrient Agar Plates for White or Cream-colored Colonies 2. Make a Gram Stain and confirm the Presence of Gram Negative Rods 3. You can use your Eyepiece Reticle to measure the Length and Diameter of these Gram Negative Rods. Record these Numbers in your Lab Notebook.
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