Problemset1SP13_000

9 to study the fgfr3 pathway in more detail and to

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9. To study the FGFR3 pathway in more detail and to understand which proteins it interacts with after its auto-phosphorylation, you decide to overexpress FGFR3 in cells. What kind of mouse strain would you need to produce (select one)? A. A transgenic mouse carrying an additional copy of the human FGFR3 gene B. A mouse that is a homozygous knock-out of fgfr3 gene C. A knock-in mouse, in which the mouse fgfr3 gene is replaced with the human FGFR3
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D. A mouse that carries a “floxed” FGFR3 exon and a transgene for Cre recombinase under chondrocyte-specific promoter 10. Indicate whether the following conditions would likely cause (A) proliferation of chondrocytes, (B) inhibition of chondrocyte proliferation, or (C) no effect on chondrocyte proliferation (answers may be used more than once, not all answers might apply): 1. Antibody that inhibits FGFR3 receptor homodimerization. 2. Intravenous administration of the FGFR3 ligand. 3. FGFR3 mutation that prevents receptor autophosphorylation. 4. Constitutive activation of the Ras protein. 11. You would like to generate a liver-specific knock-out of gene X. You’ve generated a mouse that contains a floxed exon 10 of gene X (it is a large 5kb exon) on one chromosome and a deletion of gene X on the other chromosome. The mouse also carries Cre under the control of a liver-specific promoter. Gene X is normally expressed in the liver and the pancreas and produces
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9 To study the FGFR3 pathway in more detail and to...

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