With 1 drop 6 naocl and measured the absorbance first

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with 1 drop 6% NaOCl and measured the absorbance first every 10 seconds for 5 minutes, then every 30 seconds for 15. In conclusion, the relationship between absorption versus concentration was very constant, and the ε values that were calculated were very similar, except for the most concentrated 0.001M solution of each dye. Due to miscommunication in the first calibration curve part of the experiment, our absorption measurements were taken at the λ max of each concentration of dye rather than at a constant wavelength, which means we can’t graph the wavelength vs. absorbance. Our absorption vs. time graph, although all of the steps were followed, does not seem to have any pattern to it which means something may have been messed up in the setting up of the reaction itself. My percent error of the ε Data: For allura red: 2
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Concentration Absorbance λ max ε Calculations: 2.96=0.001*1*ε, 2.96/0.001=ε, ε=2960 1.13=0.0001*1*ε, 1.13/0.0001=ε, ε=11300 0.81=0.00008*1*ε, 0.81/0.00008=ε, ε=10125 0.57=0.00005*1*ε , 0.57/0.00005=ε, ε=11400 0.36=0.00003*1*ε, 0.36/0.00003=ε, ε=12000 0.1=0.00001*1*ε, 0.1/0.00001=ε, ε=10000 Mean value of ε: 2960 + 11300 + 10125 + 11400 + 12000 + 10000 6 =9630 Mean value of λ max : 432 + 500 + 501 + 498 + 503 + 508 6 =490 0.001M 2.96 432 2960 0.0001M 1.13 500 11300 0.00008M 0.81 501 10125 0.00005M 0.57 498 11400 0.00003M 0.36 503 12000 0.00001M 0.1 508 10000 For erioglaucine (blue) Concentration Absorbance λ max ε Calculations: 3.22=0.001*1*ε, 3.22/0.001=ε, ε=3220 2.92=0.0001*1*ε, 2.92/0.0001=ε, ε=29200 2.84=0.00008*1*ε, 2.84/0.00008=ε, ε=35500 2.51=0.00005*1*ε , 2.51/0.00005=ε, ε=50200 1.67=0.00003*1*ε, 1.67/0.00003=ε, ε=55666 0.55=0.00001*1*ε, 0.55/0.00001=ε, ε=55000 Mean value of ε: 3220 + 29200 + 35500 + 50200 + 55666 + 55000 6 =228786 Mean value of λ max : 580 + 618 + 619 + 627 + 630 + 629 6 =617 0.001M 3.22 580 2960 0.0001M 2.92 618 11300 0.00008M 2.84 619 10125 0.00005M 2.51 627 11400 0.00003M 1.67 630 12000 0.00001M 0.55 629 10000 3
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  • Fall '06
  • Goddfried
  • Mean, 175, Erioglaucine Dye

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