E amylovora characterization d barionovi et al 1092

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E. amylovora characterization D. Barionovi et al. 1092 Journal compilation ª 2006 The Society for Applied Microbiology, Journal of Applied Microbiology 100 (2006) 1084–1094 ª 2006 The Authors
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UK (Jock et al. 2003). No other isolates were found in Europe with this specific number of SSR units. Tracea- bility of strains through SSR numbers is limited to dis- tinct SSR numbers, (very low or very high SSRs) (Ruppitsch et al. 2004). However, our results support the assumption that most Italian E. amylovora strains isolated in the Po valley originated from infected plant material from Belgium (Zhang et al. 1998; Jock et al. 2002). In fact, nearly all Italian strains used in this study had SSR numbers of four to six SSR units similar to strains from Belgium (Jock et al. 2003). Therefore, strain UniBaBA6 with eight SSR units and isolated from Apu- lia region in southern Italy, might have another origin. Strains with that SSR number have been previously found in Germany, England, the Netherlands, Austria, Hungary, the USA and Italy (Kim and Geider 1999; Jock et al. 2003; Ruppitsch et al. 2004). The same number of SSR units was found in this study for the Hungarian strains were formerly determined by Kim and Geider (1999). This shows that the SSR number is a suitable typing method for strains at least under laboratory con- ditions. No other correlation due to the SSR number of the investigated strains was observed. Acknowledgements This work was financed by the Italian Ministry of Agri- culture and Forestry in the framework of the project: ‘PRIA-Ricerche sul pero finalizzate alla riduzione dell‘impatto ambientale e alla valorizzazione della qua- lita `’, Publication No. 15. The authors wish to thank the following colleagues for having supplied E. amylovora and E. pyrifoliae strains. C. Bazzi (University of Bologna, Bologna, Italy), C. Cariddi (University of Bari, Bari, Italy), V. Catara (University of Catania, Catania, Italy), V. Kudela (Institute of Plant Molecular Biology, Ceske Budjovice, Czech Republic), J.D. Janse (Plant Protection Service, Wageningen, the Netherlands), M.M. Lopez (Instituto Valenciano de Investigaciones Agrarias, Moncada-Valencia, Spain), C. Manceau (INRA- Pathol- ogie Ve ´ge ´tale, Beaucouze ´, France), J. Ne ´meth (Plant Health and Soil Conservation Service, Pecs, Hungary), P. Psallidas (Benaki Phytopathological Institute, Kifissia- Athens, Greece), D.E. Stead (DEFRA Central Science Laboratory, York, UK). The authors wish to thank Dr B. Duffy, Swiss Federal Research Institute for Fruit Pro- duction, Viticulture and Horticulture, Wadenswil, Swit- zerland, for critical reading of the manuscript. References Bereswill, S., Pahl, A., Bellemann, P., Zeller, W. and Geider, K. (1992) Sensitive and species-specific detection of Erwinia amylovora by PCR-analysis. Appl Environ Microbiol 58 , 3522–3526. Bereswill, S., Bugert, P., Bruchmuller, I. and Geider, K. (1995) Identification of the fire blight pathogen, Erwinia amylovo- ra, by PCR assay with chromosomal DNA. Appl Environ Microbiol 61 , 2636–2642.
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  • Spring '08
  • Devartanian
  • DNA, DNA sequencing, Restriction enzyme, Greece Greece Greece Greece Greece Greece Greece Greece Hungary Hungary Hungary Hungary Hungary Hungary Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy USA USA Italy Italy Italy Italy, E. amylovora

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