Phenols exert their germicidal effect by denaturing

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Phenols exert their germicidal effect by denaturing proteins and destroying the selective permeability of the cell membrane (which makes cells leak). Image: Lysol on shelf , Bnilsen From the Virtual Microbiology Classroom on ScienceProfOnline.com
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D. Chemical Microbial Control 1. Materials: a. The chemical agent and type of bacteria that you were assigned. b. 10 tubes of broth c. 10 blank (empty) tubes 2. Label 1 blank and 1 broth tubes with dilution & type of bacteria: a. label strength (LS) = one blank and two broth tube b. 10-1 = one blank and one broth tube c. 10-2 = one blank and one broth tube d. 10-3 = one blank and one broth tube e. 10-4 = one blank and one broth tube 3. Working with the blank tubes, pipet 10 ml of each of the agent dilutions (i.e. label strength, 10-1, 10-2…) into the appropriately labeled empty tube. 4. Note the time, then inoculate the five corresponding blank tubes that now contain the various dilutions with one loopful of E. coli or Bacillus. 5. After 10 minutes , aseptically transfer one loopfull from the agent dilution tube labeled "label strength" to a culture broth tube that is appropriately labeled. 6. Repeat this step for each of the dilutions. Make sure that you are sterilizing your loop between each transfer!! 7. Place the serial dilution tubes (the “blanks”) in the DISCARD rack on the side bench. 9. Place the inoculated broth tubes in the “SAVE” labeled green bin for incubation and storage until next week when we will determine the patterns of growth. LS LS 10 min 10-1 10-1 10-2 10-2 Original chemical solution + inoculation 5 Blanks 5 Broths Set up for E. coli and Bacillus 10-3 10-4 10-3 10-4 From the Virtual Microbiology Classroom on ScienceProfOnline.com
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Confused? Here are links to fun resources that further explain microbial control: Microbial Control Laboratory Main Page on the Virtual Microbiology Classroom of Science Prof Online . Control of Microbial Growth , Todar’s Online Textbook of Bacteriology. Play Pandemic 2 a video game of strategy, where you try to become a successful pandemic microbe and infect the world. My 14-year old, crazy-smart daughter recommends this one to you. Play Disease Defenders educational video game, Rice University. Pasteur’s Experiment in which he used heat to control microbes animation and quiz from WH Freeman. (You must be in PPT slideshow view to click on links.) From the Virtual Microbiology Classroom on ScienceProfOnline.com
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Are microbes intimidating you? Do yourself a favor. Use the… Virtual Microbiology Classroom ( VMC ) ! The VMC is full of resources to help you succeed, including: practice test questions review questions study guides and learning objectives You can access the VMC by going to the Science Prof Online website Images: E. coli, Giant Microbes; Prokaryotic cell , Mariana Ruiz
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