Solution Couple gene for antibiotic resistance to gene being moved Creation of

Solution couple gene for antibiotic resistance to

This preview shows page 15 - 29 out of 64 pages.

Solution: Couple gene for antibiotic resistance to gene being moved
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Creation of Genetically Engineered Plants - Prerequisites 1. Growth of individual plant cells (protoplast or tissue culture) 2. Regeneration of entire plants from protoplasts or tissue cultures 3. Alteration of nucleus of plant cell by inserting new genetic material: - Plasmid, carried by Agrobacterium - mechanically, by using “gene gun” 4. Screen for transformed plants use antibiotic resistance: Problem: How to detect the 1 in 1000’s that is transformed? Solution: Couple gene for antibiotic resistance to gene being moved Treat growing cell cultures with antibiotic survivors transformed
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Creation of Genetically Engineered Plants - Prerequisites 1. Growth of individual plant cells (protoplast or tissue culture) 2. Regeneration of entire plants from protoplasts or tissue cultures 3. Alteration of nucleus of plant cell by inserting new genetic material: - Plasmid, carried by Agrobacterium - mechanically, by using “gene gun” 4. Screen for transformed plants use antibiotic resistance: Problem: How to detect the 1 in 1000’s that is transformed? New problem: does this spread antibiotic resistance?
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Bt Bt Bacillus thuringiensis common soilborne bacterium
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Bt Bt Bacillus thuringiensis common soilborne bacterium Produces proteins (“crystal proteins”, Cry) that selectively kill certain groups of insects - stomach toxins, must be ingested to kill
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Bt Bt Bacillus thuringiensis common soilborne bacterium Produces proteins (“crystal proteins”, Cry) that selectively kill certain groups of insects - stomach toxins, must be ingested to kill - protein binds to receptors in intestines insect stops eating - used in granular or liquid form > 30 years as a pesticide
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Bt Bt Bacillus thuringiensis common soilborne bacterium Produces proteins (“crystal proteins”, Cry) that selectively kill certain groups of insects - stomach toxins, must be ingested to kill - protein binds to receptors in intestines insect stops eating - used in granular or liquid form > 30 years as a pesticide - many (>60) different Cry proteins effective against different insects
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Central Dogma of Molecular Biology DNA RNA polypeptides enzymes chemical reactions life DNA DNA
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Central Dogma of Molecular Biology DNA RNA polypeptides enzymes chemical reactions life DNA DNA Analyze amino acid sequence
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Central Dogma of Molecular Biology DNA RNA polypeptides enzymes chemical reactions life DNA DNA Analyze amino acid sequence Deduce RNA, DNA sequence Find gene
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Advances in Molecular Genetics Gene coding sequence (for polypeptide)
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Advances in Molecular Genetics Gene coding sequence (for polypeptide) Promoter controls where and how much protein is made
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Advances in Molecular Genetics Gene coding sequence (for polypeptide) Promoter controls where and how much protein is made (often): genes for detection antibiotic resistance, herbicide resistance Combination of gene + promoter + detecter = cassette
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