14 follow the teachers instructions to turn on the

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Chemistry for Today: General, Organic, and Biochemistry
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Chapter 20 / Exercise 20.19
Chemistry for Today: General, Organic, and Biochemistry
Seager/Slabaugh
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14. Follow the teacher’s instructions to turn on the power supply. 15. Check the DNA samples 5 minutes after turning on the power supply. Make sure the loading dye is migrating out of the well and moving towards the positive pole. 16. Check your gel every ten minutes and turn off the power supply when the dye is near the bottom of the gel. The gel should take 30-45 minutes to run. During this time, continue work on the Student Resource Sheet. 17. When your gel is finished running, follow your teacher’s instructions to stain and visualize the DNA fragments. 18. Examine your results and finish the conclusion section.
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Chemistry for Today: General, Organic, and Biochemistry
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Chapter 20 / Exercise 20.19
Chemistry for Today: General, Organic, and Biochemistry
Seager/Slabaugh
Expert Verified
A.1.3.1 © 2009 Project Lead The Way, Inc. HBS Activity 1.3.1 DNA Detectives – Page 7 Conclusion Questions: 1. Explain the relationship between the following words – cells, genes, chromosomes, tissues, DNA, proteins. 2. Explain why scientists probably used PCR when they prepared the DNA for your experiment. 3. In the space below, explain what you would say to the other family to convince them that the science techniques used prove the bones do not belong to their loved one. 4. Suggest reasons why it was helpful to digest each of your samples with two different restriction enzymes? How do the results of your gel reinforce this point? 5. Explain how the code in your DNA relates to your physical appearance as well as the functioning of your body. How might a change in this code impact the body? 6. Other than information from bone and from DNA analysis, what other characteristics/identifiers can be used to identify this skeleton? 7. Analyze the pattern of fragments that you see. Do the DNA fingerprints from the bone sample match up with either of the missing persons? * Remember to complete all sections per the Rubric you were handed during activity 1.2.3. This Case Report will count as a Summative grade!

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