In the late 1950s meselson and stahl grew bacteria in

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23) In the late 1950s, Meselson and Stahl grew bacteria in a medium containing "heavy" nitrogen ( 15 N) and then transferred them to a medium containing 14 N. Which of the results in Figure 16.1 would be expected after one round of DNA replication in the presence of 14 N? Answer: D Topic: Concept 16.2 Skill: Application/Analysis 24) A space probe returns with a culture of a microorganism found on a distant planet. Analysis shows that it is a carbon-based life-form that has DNA. You grow the cells in 15 N medium for several generations and then transfer them to 14 N medium. Which pattern in Figure 16.1 would you expect if the DNA was replicated in a conservative manner? Answer: B Topic: Concept 16.2 Skill: Application/Analysis 25) Once the pattern found after one round of replication was observed, Meselson and Stahl could be confident of which of the following conclusions? A) Replication is semi-conservative. B) Replication is not dispersive. C) Replication is not semi-conservative. D) Replication is not conservative. E) Replication is neither dispersive nor conservative. Answer: D Topic: Concept 16.1 Skill: Knowledge/Comprehension 26) An Okazaki fragment has which of the following arrangements? 7
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27) In E. coli , there is a mutation in a gene called dnaB that alters the helicase that normally acts at the origin. Which of the following would you expect as a result of this mutation? 28) Which enzyme catalyzes the elongation of a DNA strand in the 5' 3' direction? 29) What determines the nucleotide sequence of the newly synthesized strand during DNA replication? A) the particular DNA polymerase catalyzing the reaction B) the relative amounts of the four nucleoside triphosphates in the cell C) the nucleotide sequence of the template strand D) the primase used in the reaction E) the arrangement of histones in the sugar phosphate backbone Answer: C Topic: Concept 16.2 Skill: Knowledge/Comprehension
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