Depth of focus is the range of depth that the object is still in adequate focus

Depth of focus is the range of depth that the object

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4) What is Köhler illumination and why is important? List the steps required to adjust the microscope for Köhler illumination.
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5) Report in tabular form, for each of the objectives the corresponding N.Aand total magnification. Calculate the theoretical distance for which two objects can be clearly seen as separate using Abbe’s equation. (I used 6.12 in the Abbe’s equation) Objective Magnification Objective NA Ocular Total Distance (µm) Magnificatio n Magnificatio n { λ =550 nm} 4X 0.1 10X 40X 3,366 10X 0.25 10X 100X 1346.4 40X 0.65 10X 400X 517.8 100X 1.25 10X 1000X 269.3
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  • Spring '08
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  • specimen

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