The organism has also been recovered from ocular lesions in the short sunfish

The organism has also been recovered from ocular

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The organism has also been recovered from ocular lesions in the short sunfish (Mola mola) in Spain (Hispano et al., 1997). The synonymy of V. harveyi and V. carchariae was realised by Pedersen et al. (1998) as a result of phenotypic and genotypic studies, and by Gauger and Gomez-Chairri (2002) from 16D rDNA sequencing. In terms of taxonomic standing, harveyi has precedence, and therefore this name will be used in preference to carchariae.
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142 Bacterial Fish Pathogens Vibrio harveyi V. harveyi shows similarities to V. alginolyticus, principally because of the presence of swarming on agar medium. Essentially, cultures comprise pleomorphic, fermen- tative. Gram-negative rods (1.0-1.6 x 0.5-0.7 |im in size), which are motile by polar and/or lateral flagella. Catalase, indole, lysine and ornithine decarboxylase and oxidase are produced, but not arginine dihydrolase. The Voges Proskauer reaction is negative. Nitrates are reduced. Alginate, blood, DNA, gelatin and lecithin are degraded, but not aesculin, casein, cellulose, pectin or starch. Many compounds are utilised, including arabinose, aminobutyrate, cellobiose, ethanol, glucose, glycine, ot-ketoglutarate, propanol, sucrose and trehalose, but not inositol or lactose. Growth occurs in 3-8%, but not 0% or 10% (w/v), sodium chloride, and at 11-40°C. Sensitivity is demonstrated to 150 jig, but not to 10 jig, of the vibriostatic agent, 0/129. The G + C ratio of the DNA is 45-47 mol %. The only differences with the description of V. carchariae centre on the degradation of starch {V. carchariae = -^), and growth in 7% (w/v) sodium chloride (V. carchariae = —). The G + C ratio of the DNA of V. carchariae is 47 mol %. Results of DNA: DNA homology experiments showed a high degree of homology with Ph. damselae, at 88% re-association. Eight RAPD and 13 ribotypes were recognised by Pujalte et al. (2003), Phenotypic and genotypic traits point to related- ness between V. harveyi and V. campbellii (Gomez-Gil et al., 2004). Moreover, these authors considered that some cultures of V. harveyi should more correctly have been identified as V. campbellii. This raises the question about the role of V. campbellii in fish pathology. V, ichthyoenteri V. ichthyoenteri was described as a result of an examination of seven isolates from flounder larvae (Ishimaru et al., 1996). V. ichthyoenteri Cultures are non-pigmented on marine 2216E agar, but produce yellow colonies on TCBS, which contain Gram-negative, fermentative rods, which are motile by single polar flagella. Catalase and oxidase are produced, but not arginine dihy- drolase, P-galactosidase, H2S, indole or ornithine decarboxylase. Nitrates are reduced. Neither agar, chitin, gelatin, lipids nor starch are degraded. Polyhydroxy- butyrate is not accumulated intracellularly. Growth occurs at 15-30°C, but not at 4 or 35°C, and in 1-6%, but not 0 or 8% (w/v), sodium chloride. Acid is produced from fructose, D-glucose, maltose, D-mannose, sucrose and trehalose, but not from adonitol, L-arabinose, D-cellobiose, dulcitol, erythritol, D-galactose, glyc- erol, inulin, inositol, lactose, D-mannitol, melibiose, raffinose, L-rhamnose, salicin or D-sucrose. Neither D-cellobiose, citrate, D-gluconate, L-leucine nor D-xylose
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