techniques that came into play when smearing. One of my biggest aseptic technique error was the step to flame the test tube after opening and before closing. To help improve that technique I went into lab hours and focused on that specific technique while also improving my streaking technique. While smearing I observed that it was beneficial to work closer to the 1.5-foot mark so the heat wouldn’t evaporate the water or the bacteria. After successfully smearing and staining I observed the two slides under the microscope. When setting up the microscope I made sure the objective lens was set to 10 before using the eyepiece to view the slide. The difference between each objective lens is the amount of magnification each has. The first lens has a magnification of 10x, then 40x and 100x which is used for oil immersion. After reaching oil immersion I was able to view the slides. The first one I observed was Escherichia coli was violet colored and single rod. The second slide I observed was Staphylococcus aureus was violet colored, coccus and clustered together. My slides were appropriately stained and smearing giving me the chance to observe them under the microscope. In protozoa, Amoeba, Paramecium, and Euglena are all viewed with a 100-400x magnification while Algae depends on whether if it is Chorella (100x), Oocystis (700x) or Spirogyra (175x) and bacteria is viewed at 1000x.
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