# What is the definition of field of view the area you

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2. What is the definition of field of view? The area you see in the circle 3. What happens to the size of the field of view as you increase magnification? The specimen will appear larger because of the smaller area of view 4. Under which objective is the field of view the largest? 40 x (or 4x10) Resolving Power Resolving power is the ability to distinguish between two separate points that are very close together. 1. Make a dry mount using a square of solid color from a colored picture. a. What color is your square? Purple 2. Observe the slide under low, medium, and high powers. a. How is the color distributed? The color becomes darker the higher the power/lens b. What colors do you see? High – grey, medium – dark purple, low – bright purple c. Which resolving power is greater, the human eye’s or the microscope’s? Microscopes power is greater d. How do you know? Under a microscope you can see things the human eye could never fathom to see. Depth of Field Remember that specimens have depth (thickness) and are not flat, and the whole specimen is not in one plane (on one level). The microscope only focuses on one level at a time, so you will only be able to focus on one part of the specimen at a time. 1. Make another dry mount slide using newsprint with multiple colors slide under low power. Focus directly on the point where the hairs cross. Make certain that the “X” of the hairs is directly in the center of your field of view. a. Are both hairs in focus under low power? Yes 2. Switch to medium power, focus making certain that the “X” of the hairs is directly in the center of your field of view. Created by: Diane Gibson

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3. Switch to high power. Once again focus making certain that the “X” of the hairs is directly in the center of your field of view. a. Are both hairs in focus under high power? No Why not? The microscope is only able to focus on one hair at a time Draw the hairs as viewed under high power in color. 1. Place a drop of pond water in the middle of your slide.
• Fall '19
• low power, Diane Gibson

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