Dna to renature but not the chromosomal dna which

(b)How are proteins removed from the plasmid DNA isolation? Typically during ethanol
Exercise 4 Page of
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Group Members: _______Ben Kensing Clark Plost Nicole Tran Kevin Yee_________Exercise__4___Date: __2/16/14____
LI _____________________________ Unique __________ Day: Wednesday Room: _______ Lab Start Time 6
(c)Define what a restriction enzyme is and what it does. What is the DNA sequence that the
restriction enzyme EcoR I recognizes? That BpmI recognizes?
(d)Describe the size and structure of pLAF including the function of the various components.
Compare the natural function from how components of the lac operon such as the lac promoter
and the lac-Z gene are utilized in biotechnology and cloning experiments
(e)What are the functions of each of the components of the “6 X DNA Loading dye” solution?
(2) I
NDIVIDUAL
: Library Modules
As part of Exercise 06 you will give a presentation to your class concerning a protein of your
choice. To prepare and help you gather information about your protein of interest, complete the six
library modules. (Links also on the course website under Lab Resources.)