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(b)How are proteins removed from the plasmid DNA isolation? Typically during ethanol Exercise 4 Page of 47
Group Members: _______Ben Kensing Clark Plost Nicole Tran Kevin Yee_________Exercise__4___Date: __2/16/14____ LI _____________________________ Unique __________ Day: Wednesday Room: _______ Lab Start Time 6 (c)Define what a restriction enzyme is and what it does. What is the DNA sequence that the restriction enzyme EcoRI recognizes? That BpmIrecognizes? (d)Describe the size and structure of pLAFincluding the function of the various components. Compare the natural function from how components of the lac operon such as the lac promoter and the lac-Z gene are utilized in biotechnology and cloning experiments (e)What are the functions of each of the components of the “6XDNA Loading dye” solution?(2)INDIVIDUAL:Library Modules As part of Exercise 06 you will give a presentation to your class concerning a protein of your choice. To prepare and help you gather information about your protein of interest, complete the six library modules. (Links also on the course website under Lab Resources.)