Nonprotein Nitrogen Compounds Objectives

Methods are used most frequently enzyme urease

Info icon This preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
methods are used most frequently: enzyme urease hydrolyzes urea in the sample; urea measurement can be measured in plasma, serum, or urine, avoid sodium citrate and sodium fluoride b/c they inhibit urease , refrigerate – urea is susceptible to bacteria decomposition Creatinine --- used to determine sufficiency of kidney function and the severity of kidney damage and to monitor the progression of kidney disease; plasma creatinine concentration is a function of relative muscle mass, the rate of creatinine turnover, and renal function; elevated creatinine concentration is associated w/ abnormal renal function especially as it relates to glomerular filtration – plasma concentration of creatinine is inversely proportional to clearance of creatinine therefore when plasma creatinine concentration is elevated , GFR is decreased , indicating renal damage , plasma creatinine is a relatively insensitive marker and may not be measurably increased until renal function has deteriorated more than 50%; Specimen : plasma, serum, urine – avoid hemolysis and icteria , interferences cephalosporine antibiotics and dopamine
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
NONPROTEIN NITROGEN COMPOUNDS OBJECTIVES Creatinine Clearance --- a measure of the amount of creatinine eliminated from the body by the kidneys or the volume of plasma from which creatinine is removed per unit of time ; sometimes used w/ GFR to asses renal dysfunction; Calculations: CrCl=Ucr x Vml/Pcr x Tmin Ucr = urine creatinine in mg/dl Vml = total volume in ml Pcr = plasma creatinine in mg/dl Tmin = time of collection in minutes Can be corrected for body surface area CrCl = (Ucr x Vml/ Pcr x Tmin) x (1.73/BSA) Uric Acid --- breakdown product of purine metabolism ; relatively insoluble in plasma and at high concentrations can be deposited in the joints and tissue causing painful
Image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern