6 2 In vitro hemolysis cont 9 Freeze thawing of whole blood 10 Centrifuging the

6 2 in vitro hemolysis cont 9 freeze thawing of whole

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2-In vitro-hemolysis (cont.): 9- Freeze- thawing of whole blood. 10- Centrifuging the blood before completion of coagulation. 11- Centrifugation of partially coagulated specimens from patients on anticoagulants. 12- Storage or transport of whole blood over several days at ambient temperatures. 7
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Preventing hemolysis during Specimen Collection and handling: 1. Use a 20-22 gauge needle for routine collection; too small needle results in excess vacuum force, while too large needle can cause shear stress on the cell walls. 2. Warm up the puncture site; warming increases the blood flow and prevents the need to “milk” the site, a significant cause of hemolysis. 3. Do not leave the tourniquet on for longer than one minute; prolonged tourniquet time causes the interstitial fluid to leak into the tissue, promoting hemolysis. 4. Alcohol damages cell walls; allow the venipuncture site to completely air dry after cleaning it with alcohol. 8
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Preventing hemolysis during Specimen Collection and handling: 5- Place the needle correctly in the vein; if the bevel of the needle is crowded by the inner wall of the vein, the partial occlusion exerts a dramatic shear force on the cells. This is typically indicated by too slow a blood flow.
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  • Winter '20
  • Dr. Majed Saeed
  • Hematology, Hemoglobin, specimen collection

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