What changes in global chromatin organization

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What changes in global chromatin organization accompany cell differentiation (and stem cell re- programming)? What genes are found at specific subnuclear domains ?
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Conventional TEM of the nucleus Contrast achieved with heavy atom stains (e.g. Uranium salts) Alberts-- Molecular Biology of the Cell
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P N N-P P and N mapping of a 16-cell mouse embryo nucleus by Electron Spectroscopic Imaging (ESI) 400 nm Kashif Ahmed
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P and N maps of nucleolus and surrounding nucleoplasm (SK-N-SH neuroblastoma) Ren Li
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P and N maps of nucleolus and surrounding nucleoplasm
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Interchromatin domain model Branco et al. 2006 PLoS (Anna Pombo lab) Kosak and Groudine 2004 Genes Dev. 18: 1371-1384 Interchromosomal network model Widely differing chromatin configurations-- a single model does not describe all cell types. Mouse ES cell Hematopoietic progenitor Hepatocyte Kidney epithelium Mature B cell Mature B cell Kashif Ahmed
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E3.5 Embryo- Cells with differing fates express unique factors Nanog (epiblast) Gata6 (primitive endoderm) Cdx2 (Trophectoderm) Rossant lab
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Like pluripotent blastocyst cells, ESCs have widely dispersed chromatin, little or no compaction at the nucleolus periphery and no compaction of constitutive heterochromatin chromocentre domains Dispersed chromatin configuration in e3.5 blastocysts and ESCs ESC Calvin Tang 500 nm
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Cell 1 Cell 2 Similar chromatin configuration in e5.5 epiblast cells and EpiSCs pluripotent ”, but “primed for differentiation” Epiblast EpiSC (tissue culture model) Kashif Ahmed, Peter Rugg-Gunn, Calvin Tang
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