The actual number of coliform bacteria 95 confidence

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• The actual Number of Coliform Bacteria (“95% Confidence Limit”) is between 30 and 380 Coliform Bacteria per 100 ml of Water. _______________________________________________________________ C. • The Most Probable Number (MPN) of Total Bacteria in this Water Sample is 210 Bacteria per Milliliter of Water. • The Number of Bacteria (“95% Confidence Limit”) is between 35 and 470 Coliform Bacteria per Milliliter of Water. _______________________________________________________________ D. • The Most Probable Number (MPN) of Total Bacteria in this Water Sample is 240 Bacteria per 100 ml of Water. • The Number of Bacteria (“95% Confidence Limit”) is between 36 and 1300 Bacteria per 100 ml of Water.
MIC 103L Exit Exam (Form B)Page 1013. In addition to the LOMO Microscope, your Loony Uncle in Needles also sent you a LOMO Hemocytometer (этофантастика!). Your LOMO Hemocytometer has two Counting Chambers, each of which is divided (like “Hollywood Squares”) into 9 Large Squares (1.0 mm x 1.0 mm). The Central Large Square is subdivided into 25 Medium Squares (0.2 mm x 0.2 mm; 5 Squares across and 5 Squares down). Each Medium Square is in turn subdivided into 16 Small Squares (which you ignore). A Special, Thicker-than-Normal Cover Slip creates a Vertical Distance of 0.1 mm between the Top of the Counting Chamber and the Bottom of the Cover Slip. You sterilize your Loop and scoop-up allof a small, isolated Yeast Colony to make a Yeast Suspension in exactly 5.0 ml of Sterile Saline. You use this Suspension to load your LOMO Hemocytometer. The Center-most Square -- which fills your Field of View under 10X -- is further marked-off into 25 Medium Squares (0.2 mm x 0.2 mm).You count the Number of Yeast Cells in 4 of these 25 Medium Squares (the Medium Squares in each Corner). Your Counts are: 47, 36, 45 and 32. Approximately how many Yeast Cells were there in this entire small, isolated Yeast Colony that you scooped-up to make your Yeast Suspension? A. 1.0 x 107Yeast Cells___________________________ B. 3.2 x 107Yeast Cells___________________________ C. 4.0 x 107Yeast Cells___________________________ D. 5.0 x 107Yeast Cells
MIC 103L Exit Exam (Form B)Page 1114. You and your Lab Partner obtain a 1.0 Kg Sample of Ground Beast from Bwana Billʼs Safari Burger. You add 10 Grams of Ground Beast to 90 ml of Sterile Saline and blend this on “High” for 30 Seconds. You then use this 10-1Suspension to set-up a Viable Count Dilution Series, exactly as we did in Lab. When you have completed setting-up your Dilution Series, it will consist of -- A. Six Test Tubes, each with 4.5 ml of a different Dilution. _________________________________________________________________ B. Six Test Tubes, each with 5.0 ml of a different Dilution. _________________________________________________________________ C. • Five Test Tubes, each 4.5 ml of a different Dilution • One Test Tube with 5.0 ml of a different Dilution _________________________________________________________________ D. • Five Test Tubes, each with 5.0 ml of a different Dilution • One Test Tube with 4.5 ml of a different Dilution

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