RE & Complementation

Met ura glu no significant growth was observed table

Info icon This preview shows pages 7–9. Sign up to view the full content.

View Full Document Right Arrow Icon
I. -met –ura +glu, no significant growth was observed. Table 3: Complementation of met genes by yeast overexpression plasmids S. cerevisae transformed with pYES2.1- lacZ S. cerevisae transformed with pYES2.1+ Met3 S. cerevisae transformed with pBG1805+ MET3 +met -ura +glu Baseline growth Significant growth Significant growth -met -ura +gal No growth Significant growth Significant growth -met -ura +glu No growth No growth No growth Conclusions Observed band sizes for plasmid 4 match the expected band sizes for pYES2.1- lacZ , suggesting plasmid 4 is pYES2.1- lacZ . Observed band sizes for plasmid 10 match the expected band sizes for pYES2.1+ Met3 , suggesting plasmid 10 is pYES2.1+ Met3 . Observed band sizes for plasmid 14 match the expected band sizes for pBG1805+ MET3 , suggesting plasmid 14 is pBG1805+ MET3 . YMP 12 yeast transformed with pYES2.1- lacZ grew in repressed conditions of YC +met –ura +glu media, not in YC +glu –ura –met, and not in YC +gal –ura –met, suggesting pYES2.1- lacZ does not complement the met3 auxotrophy. YMP 12 yeast auxotrophs grew in repressed conditions of YC +met –ura +glu media, not in YC +glu –ura –met , but also in YC +gal –ura –met after transformation with pBG1805+ MET3 , suggesting that MET3 complements the met3 auxotrophy and that YMP 12 is the met3 mutant. YMP 12 yeast auxotrophs grew in repressed conditions of YC +met –ura +glu media, not in YC +glu –ura –met , but also in YC +gal –ura – met after transformation with pYES2.1+ Met3 , suggesting that the S.pombe ortholog , Met3, complements the met3 auxotrophy.
Image of page 7

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Citations 1. O'Connor, Clare. (2012) Investigations in Molecular Cell Biology . Plymouth: Hayden-McNeil. Print. 2. Amberg, D.C., Burke, D.J., and Strathern, J.N. (2005). Methods in Yeast Genetics (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY). P. 121
Image of page 8
3. "PlasMapper Version 2.0." PlasMapper . PENCE & Genome Alberta, n.d. Web. 4. "Basic Local Alignment Search Tool." BLAST: . National Library of Medicine, n.d. Web.. 5. "National Center for Biotechnology Information." National Center for Biotechnology Information, NCBI . U.S. National Library of Medicine, n.d. Web. 6. "NEBcutter V2.0." NEBcutter V2.0 . New England Bio Labs, n.d. Web.
Image of page 9
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern